Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,111,845	A→C	L702R (CTG→CGG)	yrfF ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,111,845	0	A	C	100.0%	222.2 / NA	65	L702R (CTG→CGG)	yrfF	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/39);  total (26/39)

TGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCAGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGT  >  W3110S.gb/4111777‑4111911                                                                     |                                                                   tGCGCGACAGGGTAGGATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt                                                                  >  1:3881066/1‑71 (MQ=255) tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt                                                                  >  1:3585897/1‑71 (MQ=255) tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt                                                                  >  1:262923/1‑71 (MQ=255) tGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGt                                                                  >  1:2324106/1‑71 (MQ=255)         aGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGtt                                                          <  1:2737234/71‑1 (MQ=255)                 aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc                                                  >  1:4627798/1‑71 (MQ=255)                 aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc                                                  >  1:3988841/1‑71 (MQ=255)                 aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc                                                  >  1:3239806/1‑71 (MQ=255)                 aTAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGc                                                  >  1:29272/1‑71 (MQ=255)                  tAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCt                                                 >  1:3166709/1‑71 (MQ=255)                    acgtGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCATTGGGGGTTCAGGCAGCttt                                               >  1:1214679/4‑71 (MQ=255)                    aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt                                               >  1:994867/1‑71 (MQ=255)                    aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt                                               >  1:566088/1‑71 (MQ=255)                    aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt                                               >  1:4268952/1‑71 (MQ=255)                    aCCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt                                               >  1:3048479/1‑71 (MQ=255)                    aCCTGCCCCGCAAACGTGTTATTCGATAAGTCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGTAACttt                                               >  1:1950332/1‑71 (MQ=38)                      cTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCa                                             <  1:4216643/71‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:738620/71‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:4527722/71‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:426187/71‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:1575054/71‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCata                                           <  1:3579963/70‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:1720814/69‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:3891741/69‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:3764579/69‑1 (MQ=255)                         ccGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:4418976/69‑1 (MQ=255)                                 aCGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:4135098/61‑1 (MQ=255)                                  cGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTAATAATAGGCCTg                                  >  1:3044570/1‑70 (MQ=255)                                  cGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGGTCAGGCAGCTTTCATAATAGGCCTGa                                 <  1:86691/71‑1 (MQ=255)                                      ttATTCGATAAGGCTTTCTGAAGTGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:1528187/58‑1 (MQ=255)                                        aTTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAAtctc                            >  1:3558004/1‑70 (MQ=255)                                        aTTCGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAAtctc                            >  1:3690239/1‑70 (MQ=255)                                           cGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcat                         <  1:1988353/70‑1 (MQ=255)                                           cGATAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcat                         <  1:1350919/70‑1 (MQ=255)                                             aTAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCat                                            <  1:2394995/49‑1 (MQ=255)                                             aTAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCcatcat                      <  1:3258559/71‑1 (MQ=255)                                              tAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:374184/50‑1 (MQ=255)                                              tAAGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataa                                          <  1:108928/50‑1 (MQ=255)                                                aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCataata                                        >  1:3404152/1‑50 (MQ=255)                                                aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGc                    <  1:3340571/70‑1 (MQ=255)                                                aGGCTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCg                   <  1:398244/71‑1 (MQ=255)                                                   cTTTCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCttt                                               <  1:904352/40‑1 (MQ=255)                                                     ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa                                >  1:498615/1‑53 (MQ=255)                                                     ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa                                >  1:2880214/1‑53 (MQ=255)                                                     ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGaa                                >  1:4621112/1‑53 (MQ=255)                                                     ttCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc              >  1:2248119/1‑71 (MQ=255)                                                      tCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc              <  1:1716854/70‑1 (MQ=255)                                                      tCTGAAGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc              <  1:1604826/70‑1 (MQ=255)                                                          aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCTTAATAGGCCTGAATCTCCATCATGCGAGTGCGAt           <  1:1847271/69‑1 (MQ=255)                                                          aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt           <  1:3739911/69‑1 (MQ=255)                                                          aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt           <  1:2659309/69‑1 (MQ=255)                                                          aaGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt           <  1:615844/69‑1 (MQ=255)                                                           atgggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc              <  1:44188/63‑1 (MQ=255)                                                           aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCg                   <  1:2105223/60‑1 (MQ=255)                                                           aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGAt           <  1:3612997/68‑1 (MQ=255)                                                           aGGGGTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGAc        <  1:4369091/71‑1 (MQ=255)                                                             gggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGc              <  1:208971/63‑1 (MQ=255)                                                             gggTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTACATCATGCGAGTGc              <  1:3994929/63‑1 (MQ=255)                                                               gTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTg    >  1:3184349/1‑71 (MQ=255)                                                               gTGATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTg    >  1:3384335/1‑71 (MQ=255)                                                                 gATCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTgg   >  1:965819/1‑70 (MQ=255)                                                                  aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGc                    <  1:2220695/52‑1 (MQ=255)                                                                  aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt  <  1:3192470/70‑1 (MQ=255)                                                                  aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt  <  1:3532730/70‑1 (MQ=255)                                                                  aTCCGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGt  <  1:3611191/70‑1 (MQ=255)                                                                     |                                                                   TGCGCGACAGGGTAGCATAACCTGCCGCGCAAACGTGTTATTCGATAAGGCTTTCTGAAGGGGTGATCAGTTGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCTGGT  >  W3110S.gb/4111777‑4111911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑