Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,182,652	C→T	V464M (GTG→ATG)	gspD ←	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,182,652	0	C	T	100.0%	144.0 / NA	48	V464M (GTG→ATG)	gspD	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

CGTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGATG  >  W3110S.gb/4182584‑4182719                                                                     |                                                                    cGTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCatat                                                                   <  1:895508/71‑1 (MQ=255)  gTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATc                                                                  >  1:2268762/1‑71 (MQ=255)     ttGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCt                                                                <  1:55215/70‑1 (MQ=255)            cGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACg                                                        >  1:1209408/1‑71 (MQ=255)                   aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAg                                                  >  1:2573267/1‑70 (MQ=255)                   aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCAGGATAGCACCGGCATATCCTGCCCCACGTTGAAg                                                  >  1:2444619/1‑70 (MQ=255)                    ttAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGa                                                >  1:1076761/1‑71 (MQ=255)                    ttAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGa                                                >  1:889672/1‑71 (MQ=255)                      aaaGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcg                                              >  1:4254315/1‑71 (MQ=255)                      aaaGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcg                                              >  1:2640147/1‑71 (MQ=255)                      aaaGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcg                                              >  1:742370/1‑71 (MQ=255)                      aaaGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcg                                              >  1:494854/1‑71 (MQ=255)                        aGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcgc                                             >  1:4000427/1‑70 (MQ=255)                         gACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcgc                                             >  1:3150257/1‑69 (MQ=255)                         gACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGAcgc                                             >  1:3269975/1‑69 (MQ=255)                            gTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGtt                                        <  1:1019700/71‑1 (MQ=255)                            gTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGtt                                        <  1:3640409/71‑1 (MQ=255)                             ttATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGttt                                       <  1:407078/71‑1 (MQ=255)                             ttATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGttt                                       <  1:1935297/71‑1 (MQ=255)                               aTCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATc                                                                  >  1:663059/1‑42 (MQ=255)                               aTCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTttat                                     >  1:556450/1‑71 (MQ=255)                                 ccacTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGAGAGtt                                        <  1:2805617/63‑1 (MQ=255)                                      gAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCa                               >  1:48224/1‑70 (MQ=255)                                      gAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAg                              >  1:3963184/1‑71 (MQ=255)                                       aaGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGc                             <  1:2708544/71‑1 (MQ=255)                                       aaGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGc                             <  1:912416/71‑1 (MQ=255)                                          tGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGtt                          <  1:2542695/71‑1 (MQ=255)                                           ggTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCAGCTTCAAGGACGCGAGTTTATTATCCAGCGTTa                         >  1:2527043/1‑71 (MQ=255)                                            gTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTAc                        >  1:2663711/1‑71 (MQ=255)                                            gTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTAc                        <  1:1369998/71‑1 (MQ=255)                                            gTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTAc                        <  1:4046191/71‑1 (MQ=255)                                             tCTGTTACCCGGATAGCACCGGCATATCCTGCCCCAc                                                         <  1:1806224/37‑1 (MQ=38)                                               tgtgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTtattat                                  >  1:219659/1‑58 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGAt                     <  1:3291471/69‑1 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATg                    <  1:144482/70‑1 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATg                    <  1:2356772/70‑1 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATg                    <  1:1888434/70‑1 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATg                    <  1:1775386/70‑1 (MQ=255)                                                 tgACCCGGATAGCACCGGCATATCCTGCCCCACGTCGAAGGCCGCGAGTTTATTATCCAGCGTTACGATGc                   >  1:2773694/1‑71 (MQ=255)                                                        gATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGa                                           <  1:1997254/40‑1 (MQ=255)                                                          tAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTgg          >  1:4181038/1‑71 (MQ=255)                                                              aCCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg      <  1:4248678/71‑1 (MQ=255)                                                              aCCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg      <  1:4202389/71‑1 (MQ=255)                                                              aCCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg      <  1:806814/71‑1 (MQ=255)                                                              aCCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg      <  1:1873431/71‑1 (MQ=255)                                                              aCCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg      <  1:1869510/71‑1 (MQ=255)                                                               ccGGCATATCCTGCCCCACGTTGAAGGACGCGAGTttatt                                    <  1:1529353/40‑1 (MQ=255)                                                                  gCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGAt                     <  1:2651494/52‑1 (MQ=255)                                                                  gCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGATg  >  1:964190/1‑71 (MQ=255)                                                                     |                                                                    CGTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGATG  >  W3110S.gb/4182584‑4182719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑