| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,184,035 | C→A | G3* (GGA→TGA) R269M (AGG→ATG) |
gspD ← gspC ← |
general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,035 | 0 | C | A | 100.0% | 219.0 / NA | 73 | G3* (GGA→TGA) R269M (AGG→ATG) | gspD gspC | general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (38/35); total (38/35) | |||||||||||
CGTTCTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTAC > W3110S.gb/4183966‑4184099 | cgttcTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCA‑‑‑AGCAGGTGATTTTATTGAGTCAttt > 1:83114/1‑70 (MQ=255) ttcTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:991553/71‑1 (MQ=255) ttcTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:1715440/71‑1 (MQ=255) ttcTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:887023/71‑1 (MQ=255) ctcAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTc > 1:2449653/1‑70 (MQ=255) ctcAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCa > 1:4299545/1‑71 (MQ=255) ctcAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCa > 1:1898228/1‑71 (MQ=255) aGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:3860102/69‑1 (MQ=255) aGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:2804864/69‑1 (MQ=255) tgtCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:4090850/62‑1 (MQ=255) tgtCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCAttt < 1:1112438/62‑1 (MQ=255) tCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGt > 1:627736/1‑71 (MQ=255) cTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTc > 1:1515664/1‑70 (MQ=255) cTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTc < 1:360552/70‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:3941110/71‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:2453520/71‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:1090218/71‑1 (MQ=255) acaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:4037822/70‑1 (MQ=255) aaGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat < 1:2231432/71‑1 (MQ=255) ggCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCattcattc > 1:1779880/1‑71 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTc < 1:843040/47‑1 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTc < 1:3362258/47‑1 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCca < 1:3293788/60‑1 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt < 1:1181995/71‑1 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt < 1:4665707/71‑1 (MQ=255) agAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:2370200/61‑1 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGa > 1:3794697/1‑49 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACg > 1:3882547/1‑51 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt > 1:2375739/1‑60 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt > 1:1243332/1‑60 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:4334430/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:3630368/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:1231247/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:1938724/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:721326/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:64758/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:1389915/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:411890/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGt > 1:1784839/1‑69 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTa < 1:264933/70‑1 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTa < 1:823796/70‑1 (MQ=255) gtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:3425411/71‑1 (MQ=255) gtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTc > 1:4249570/1‑71 (MQ=255) gtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTc > 1:473022/1‑71 (MQ=255) gctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCa > 1:2617641/1‑40 (MQ=255) gctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCa > 1:916485/1‑40 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattca > 1:2751926/1‑55 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc > 1:2984273/1‑71 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc > 1:2854203/1‑71 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc > 1:4305858/1‑71 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACg > 1:3293919/1‑70 (MQ=255) gcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat < 1:3765363/50‑1 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTa < 1:1843350/54‑1 (MQ=255) agcagGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt < 1:643048/71‑1 (MQ=255) cagGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTa < 1:1555304/71‑1 (MQ=255) agGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTAt > 1:2970784/1‑71 (MQ=255) tGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc < 1:1269230/53‑1 (MQ=255) tGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa < 1:2660584/70‑1 (MQ=255) gaatttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:1034635/4‑71 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:1657906/1‑71 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:2013014/1‑71 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:3363896/1‑71 (MQ=255) gATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAg > 1:3190098/1‑71 (MQ=255) aTTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGc > 1:3590399/1‑71 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:921239/1‑71 (MQ=255) tttATTGAGTCATTTCATGACGCAGTACATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:4032175/1‑71 (MQ=255) aTTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:791122/70‑1 (MQ=255) aTTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:3410860/70‑1 (MQ=255) aTTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:84515/70‑1 (MQ=255) aTTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:3223972/70‑1 (MQ=255) tGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt < 1:2568266/56‑1 (MQ=255) tGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:858993/68‑1 (MQ=255) gAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTAc < 1:2500863/70‑1 (MQ=255) | CGTTCTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTAC > W3110S.gb/4183966‑4184099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |