BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,378,569	T→C	V116V (GTA→GTG)	yjeJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,378,569	0	T	C	100.0%	394.8 / NA	113	V116V (GTA→GTG)	yjeJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/67); total (46/67)

Only 100 of 113 total aligned reads displayed.

GCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAAT  >  W3110S.gb/4378502‑4378631

gcgcCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACg                                                             <  1:3261259/71‑1 (MQ=255)

gcgcCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACg                                                             <  1:1791508/71‑1 (MQ=255)

gcgcCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACg                                                             <  1:735349/71‑1 (MQ=255)

       cATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTaa                                                      <  1:2401112/71‑1 (MQ=255)

        aTTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAgg                                                         >  1:466751/1‑67 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACg                                                             <  1:4510735/62‑1 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGt                                                        >  1:2126025/1‑67 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGt                                                        >  1:4396646/1‑67 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGt                                                        >  1:1473404/1‑67 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAg                                                     <  1:1849558/70‑1 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAg                                                     <  1:2222507/70‑1 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGa                                                    <  1:1765966/71‑1 (MQ=255)

         tttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGa                                                    <  1:4168261/71‑1 (MQ=255)

          ttCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAg                                                     <  1:4313745/69‑1 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:2006080/1‑71 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:1573098/1‑71 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:259201/1‑71 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:2461132/1‑71 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:4576239/1‑71 (MQ=255)

           tCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:4230740/1‑71 (MQ=255)

            cAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAttt                                                 >  1:753219/1‑71 (MQ=255)

            cAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAttt                                                 >  1:895493/1‑71 (MQ=255)

            cAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  <  1:1350635/70‑1 (MQ=255)

              aTCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTc                                               <  1:4356377/71‑1 (MQ=255)

                cTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGc                                             >  1:910317/1‑71 (MQ=255)

                cTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGc                                             >  1:4480449/1‑71 (MQ=255)

                 tGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGcc                                            >  1:902817/1‑71 (MQ=255)

                   cAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCtt                                          <  1:2230180/71‑1 (MQ=255)

                   cAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCtt                                          <  1:2305295/71‑1 (MQ=255)

                   cAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCtt                                          <  1:587295/71‑1 (MQ=255)

                   cAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCtt                                          <  1:648829/71‑1 (MQ=255)

                   cAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCtt                                          <  1:1285754/71‑1 (MQ=255)

                    aaCTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGt                                                        <  1:3373741/56‑1 (MQ=255)

                    aaCTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGt                                                        <  1:3220607/56‑1 (MQ=255)

                    aaCTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTg                                         <  1:3843471/71‑1 (MQ=255)

                      cTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAt                                       <  1:4188867/71‑1 (MQ=255)

                      cTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAt                                       <  1:2564431/71‑1 (MQ=255)

                         aTTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAtt                                      <  1:3289490/69‑1 (MQ=255)

                         aTTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAtt                                      <  1:2745837/69‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGATGGTAAg                                                     <  1:599576/53‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAg                                                     <  1:629160/53‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTg                                         <  1:4387467/65‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTg                                         <  1:561841/65‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAtt                                      <  1:3285254/68‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGAtt                                      <  1:1969359/68‑1 (MQ=255)

                          ttGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATc                                   <  1:4461574/71‑1 (MQ=255)

                            gacgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCgg                                 >  1:3669193/1‑71 (MQ=255)

                            gacgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCgg                                 >  1:1167788/1‑71 (MQ=255)

                            gacgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCgg                                 >  1:687026/1‑71 (MQ=255)

                            gacgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCgg                                 >  1:3073203/1‑71 (MQ=255)

                              cgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTa                               <  1:1286902/71‑1 (MQ=255)

                              cgacTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTa                               <  1:3103363/71‑1 (MQ=255)

                                acTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTa                               <  1:1476001/69‑1 (MQ=255)

                                acTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAg                             <  1:858086/71‑1 (MQ=255)

                                 cTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAg                             <  1:280836/70‑1 (MQ=255)

                                 cTAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGt                            <  1:1265604/71‑1 (MQ=255)

                                  tAACTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGtt                           >  1:1985856/1‑71 (MQ=255)

                                    aCTCACATTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAg                             <  1:1994937/67‑1 (MQ=255)

                                       cacaTTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTaa                              >  1:299346/1‑63 (MQ=255)

                                        acaTTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGc                      <  1:1923827/70‑1 (MQ=255)

                                        acaTTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGc                      <  1:3169672/70‑1 (MQ=255)

                                         caTTTGCTACCGTCATGTAGCGTTAACACGAAGGTAAGAtt                                                  >  1:3741404/1‑41 (MQ=255)

                                           tttGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTAt                                    >  1:264604/1‑53 (MQ=255)

                                           tttGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGtt                           <  1:183098/62‑1 (MQ=255)

                                             tGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATc                                   >  1:3813318/1‑52 (MQ=255)

                                             tGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGtt                 <  1:4413601/70‑1 (MQ=255)

                                             tGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGtt                 <  1:3771413/70‑1 (MQ=255)

                                             tGCTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGtt                 <  1:344938/70‑1 (MQ=255)

                                               cTACCGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTaa               <  1:1359183/70‑1 (MQ=255)

                                                  ccGTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcg           <  1:2233875/71‑1 (MQ=255)

                                                    gTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTaa               >  1:2684841/1‑65 (MQ=255)

                                                    gTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAAccc            >  1:852395/1‑68 (MQ=255)

                                                    gTCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgac         >  1:2656860/1‑71 (MQ=255)

                                                     tCATTTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgac         <  1:4387246/70‑1 (MQ=255)

                                                     tCATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgacg        <  1:3095058/71‑1 (MQ=255)

                                                      cATGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCg                     >  1:1299598/1‑57 (MQ=255)

                                                       aTGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgacga       <  1:2859792/70‑1 (MQ=255)

                                                       aTGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgacga       <  1:3152808/70‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCcgacga       <  1:3323695/69‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGAtt     <  1:1015429/71‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGAtt     <  1:2095854/71‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGAtt     <  1:3197769/71‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGAtt     <  1:4285885/71‑1 (MQ=255)

                                                        tGTAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGAtt     <  1:3359257/71‑1 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGtt                           >  1:3073235/1‑47 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTaa               >  1:3704878/1‑59 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   <  1:4360060/71‑1 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:1920883/1‑71 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:3976784/1‑71 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:2140659/1‑71 (MQ=255)

                                                          tAGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:3336545/1‑71 (MQ=255)

                                                           aGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:2192744/1‑70 (MQ=255)

                                                           aGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:1787512/1‑70 (MQ=255)

                                                           aGCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   >  1:1572588/1‑70 (MQ=255)

                                                            gCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAat  <  1:3216136/70‑1 (MQ=255)

                                                            gCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAat  <  1:325119/70‑1 (MQ=255)

                                                            gCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAat  <  1:3399529/70‑1 (MQ=255)

                                                            gCGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAat  <  1:3768765/70‑1 (MQ=255)

                                                             cGTTAACACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTaa   <  1:1641986/68‑1 (MQ=255)

                                                              gTTAACTCGAAGGTAAGATTTTCGCCTTGATTATCGGTa                               <  1:67229/39‑1 (MQ=38)

GCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAAT  >  W3110S.gb/4378502‑4378631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`