BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,547,129	G→A	M137I (ATG→ATA)	fimE →	tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,547,129	0	G	A	100.0%	348.9 / NA	116	M137I (ATG→ATA)	fimE	tyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (74/42); total (74/42)

Only 100 of 116 total aligned reads displayed.

CCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATGTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAT  >  W3110S.gb/4547065‑4547192

ccTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           <  1:1774457/71‑1 (MQ=255)

ccTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           <  1:1427901/71‑1 (MQ=255)

 cTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGGACCTTAACGCAGACTCATCCTCATATATTAAgg                                                           >  1:3260993/1‑70 (MQ=255)

 cTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           >  1:933601/1‑70 (MQ=255)

 cTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           >  1:423098/1‑70 (MQ=255)

 cTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           >  1:534442/1‑70 (MQ=255)

 cTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAg                                                            >  1:4585792/1‑69 (MQ=255)

    tCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATg                                                       >  1:1311985/1‑71 (MQ=255)

    tCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATg                                                       >  1:2547335/1‑71 (MQ=255)

    tCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATg                                                       <  1:3895447/71‑1 (MQ=255)

    tCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATg                                                       >  1:814031/1‑71 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGc                                                      >  1:4253175/1‑70 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCt                                                     >  1:1451034/1‑71 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCt                                                     >  1:4212794/1‑71 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCt                                                     >  1:61409/1‑71 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCt                                                     >  1:3399382/1‑71 (MQ=255)

      gCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCt                                                     >  1:727575/1‑71 (MQ=255)

         ttattCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           <  1:1641652/62‑1 (MQ=255)

         ttattCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGc                                                  <  1:550227/71‑1 (MQ=255)

           attCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCgg                                                >  1:4566956/1‑71 (MQ=255)

           attCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCgg                                                >  1:1112724/1‑71 (MQ=255)

           attCGCGATGACGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCgg                                                >  1:33192/1‑71 (MQ=255)

                cgATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:293085/71‑1 (MQ=255)

                cgATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:1792183/71‑1 (MQ=255)

                cgATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:767901/71‑1 (MQ=255)

                cgATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:1482396/71‑1 (MQ=255)

                cgATGCCGGTATTGAAGATGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:2318197/71‑1 (MQ=255)

                 gATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGa                                          <  1:4014905/71‑1 (MQ=255)

                   tGCCGGTATTGAAGCTGTAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGt                                               >  1:2515590/1‑64 (MQ=255)

                   tGCCGGTATTGAAGCTGTAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAAt                                        >  1:2888234/1‑71 (MQ=255)

                     ccGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTg                                      >  1:1245091/1‑71 (MQ=255)

                     ccGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTg                                      >  1:3734708/1‑71 (MQ=255)

                     ccGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTg                                      >  1:1485684/1‑71 (MQ=255)

                     ccGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTg                                      >  1:4218181/1‑71 (MQ=255)

                        gTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGa                                          <  1:4139189/64‑1 (MQ=255)

                          aTTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCg                                   >  1:151780/1‑69 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATTCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAg                                >  1:1709394/1‑71 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           <  1:2136456/44‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAgg                                                           <  1:1916291/44‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCa                                                         >  1:1388397/1‑46 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGt                                               <  1:2300952/56‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:3419860/60‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:4450682/60‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:4479292/60‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:1790282/60‑1 (MQ=255)

                           ttGAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGaa                                         >  1:3371862/1‑62 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTaa                                                             >  1:3421780/1‑40 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCa                                                         >  1:4258358/1‑44 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCAt                                                        >  1:2093508/1‑45 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGATTTGGCGGAGCg                              >  1:1107750/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAgag                              >  1:4596998/1‑69 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGc                               >  1:1945799/1‑70 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGc                               >  1:1894356/1‑70 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:3707971/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:519303/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:2338741/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:3921201/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:388073/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:3822656/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:2508194/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:2601004/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:4528378/1‑71 (MQ=255)

                             gAAGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCg                              >  1:1113187/1‑71 (MQ=255)

                              aaGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgt                             <  1:4292403/71‑1 (MQ=255)

                              aaGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgt                             <  1:290183/71‑1 (MQ=255)

                              aaGCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgt                             <  1:3420918/71‑1 (MQ=255)

                                gCTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGaa                                         >  1:2839920/1‑57 (MQ=255)

                                 cTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgtggt                          <  1:4349422/71‑1 (MQ=255)

                                 cTGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgtggt                          <  1:2677679/71‑1 (MQ=255)

                                  tGGAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCgtg                            >  1:2734900/1‑68 (MQ=255)

                                    gAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGc                        <  1:761477/70‑1 (MQ=255)

                                    gAACCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCa                       <  1:2093704/71‑1 (MQ=255)

                                      aCCGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGa                     <  1:2467271/71‑1 (MQ=255)

                                       ccGTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGa                     >  1:4662996/1‑70 (MQ=255)

                                         gTAACGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATAc                  >  1:3157732/1‑71 (MQ=255)

                                            aCGCAGACTCATCCTCATATATTAAGGCATGCTTg                                                   >  1:3413250/1‑35 (MQ=255)

                                            aCGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGaa                                         >  1:4481389/1‑45 (MQ=255)

                                            aCGCAGACTCATCCTCATATATTAAGGCATGCTTGCGGTTATGaa                                         >  1:1214399/1‑45 (MQ=255)

                                                   cTCATCCTCATATATTAAGGCATGCTAGCGGTTATGaa                                         >  1:3914449/1‑38 (MQ=38)

                                                    tCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:1843329/35‑1 (MQ=255)

                                                    tCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:2244773/35‑1 (MQ=255)

                                                    tCATCCTCATATATTAAGGCATGCTTGCGGTTATg                                           <  1:1194328/35‑1 (MQ=255)

                                                    tCATCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAAtt        >  1:3778124/1‑70 (MQ=255)

                                                      aTCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCa      >  1:358349/1‑70 (MQ=255)

                                                       tCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAgg    <  1:1617561/71‑1 (MQ=255)

                                                       tCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAgg    <  1:1322987/71‑1 (MQ=255)

                                                       tCCTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAgg    <  1:1063408/71‑1 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGa                     <  1:4203395/52‑1 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGa                     <  1:233311/52‑1 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGa   >  1:3098188/1‑70 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:1816515/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:2021678/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:2228589/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  <  1:1419438/71‑1 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:1369463/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:3587976/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:3350203/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:3318259/1‑71 (MQ=255)

                                                         cTCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  <  1:3172104/71‑1 (MQ=255)

                                                          tCATATATTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAt  >  1:3512792/1‑70 (MQ=255)

CCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTCATCCTCATATGTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGAT  >  W3110S.gb/4547065‑4547192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`