Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,591,162	C→A	V112V (GTG→GTT)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,591,162	0	C	A	100.0%	157.0 / NA	52	V112V (GTG→GTT)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/29);  total (23/29)

ACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGA  >  W3110S.gb/4591097‑4591229                                                                  |                                                                    aCAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGc                                                                <  1:1801186/71‑1 (MQ=255)  cAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGcc                                                               >  1:1261292/1‑71 (MQ=255)          cGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc                                                       <  1:2382570/71‑1 (MQ=255)           ggAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTTcc                                                       <  1:1745732/70‑1 (MQ=255)                    gTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCTCCTGGt                                                           <  1:53443/57‑1 (MQ=255)                     tCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTa                                            <  1:3845877/71‑1 (MQ=255)                        cAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGt                                             <  1:2968731/67‑1 (MQ=255)                         aGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAAttt                                        <  1:927035/71‑1 (MQ=255)                          ggCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTaa                                           >  1:2389959/1‑67 (MQ=255)                            cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTtgt                                      >  1:2351559/1‑70 (MQ=255)                             acaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGa                                    <  1:4675918/71‑1 (MQ=255)                               aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  <  1:2707932/71‑1 (MQ=255)                               aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  <  1:2212418/71‑1 (MQ=255)                                tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaat                                  >  1:4269730/1‑69 (MQ=255)                                tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  >  1:4273112/1‑70 (MQ=255)                                tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  >  1:2772684/1‑70 (MQ=255)                                    ggCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa                              <  1:445980/70‑1 (MQ=255)                                       aTCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  >  1:764642/1‑63 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa                                  <  1:3878469/62‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:2669256/71‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:598110/71‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:3648955/71‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa                              <  1:4028940/66‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa                              <  1:2908071/66‑1 (MQ=255)                                        tCGTTGAGATCGTTATGATATTAGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:4395520/71‑1 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGGCTGGTTACCaa                                                     >  1:4243327/1‑41 (MQ=38)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa                                >  1:4565574/1‑62 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa                                >  1:4190123/1‑62 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACaga                            >  1:4561471/1‑66 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACaga                            >  1:3893940/1‑66 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAgg                        <  1:141180/70‑1 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:3316394/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:2021267/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:4396856/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:1254367/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:426693/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:122234/1‑71 (MQ=255)                                          gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       >  1:1052732/1‑71 (MQ=255)                                           ttGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa                       <  1:1101971/70‑1 (MQ=255)                                           ttGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAt                      <  1:3090644/71‑1 (MQ=255)                                             gagaTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtg                     <  1:2912929/70‑1 (MQ=255)                                              agaTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgtt                   <  1:1567866/71‑1 (MQ=255)                                                aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:4433714/63‑1 (MQ=255)                                                aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgttgt                 <  1:3212059/71‑1 (MQ=255)                                                aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgttgt                 <  1:56308/71‑1 (MQ=255)                                                  cGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtca               >  1:4508596/1‑71 (MQ=255)                                                      aTGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATcaa           >  1:385884/1‑71 (MQ=255)                                                        gATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:2353710/55‑1 (MQ=255)                                                          tatTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag                         <  1:2829167/53‑1 (MQ=255)                                                           atTCGTGAACCGCC‑GGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAgg     >  1:3155786/1‑71 (MQ=255)                                                              cGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGc    >  1:3863301/1‑70 (MQ=255)                                                               gTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGa  <  1:3436559/71‑1 (MQ=255)                                                                  |                                                                    ACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGA  >  W3110S.gb/4591097‑4591229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑