Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,615,718	C→T	intergenic (+33/‑360)	prfC → / → osmY	peptide chain release factor RF‑3/periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,615,718	0	C	T	100.0%	125.9 / NA	44	intergenic (+33/‑360)	prfC/osmY	peptide chain release factor RF‑3/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/16);  total (28/16)

ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT  >  W3110S.gb/4615654‑4615784                                                                 |                                                                   aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCa                                                              <  1:556310/71‑1 (MQ=255)   gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCaa                                                             <  1:1045781/70‑1 (MQ=255)      cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTTCGCTCTGTCAATTTc                                                         >  1:3701062/1‑71 (MQ=255)      cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTc                                                         >  1:4331590/1‑71 (MQ=255)       aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTc                                                         <  1:3515938/70‑1 (MQ=255)           ccaccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCccttc                                                    >  1:2149544/1‑71 (MQ=255)           ccaccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCccttc                                                    >  1:1614161/1‑71 (MQ=255)                          gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTccc                                                       >  1:2257165/1‑53 (MQ=255)                          gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTccc                                                       >  1:471737/1‑53 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGcc                                          <  1:1343582/63‑1 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                  <  1:1963791/71‑1 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                   >  1:1631731/1‑70 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                   >  1:963645/1‑70 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                   >  1:4380838/1‑70 (MQ=255)                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCACTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCt                                       <  1:1145203/66‑1 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCccttcct                                                  >  1:530414/1‑53 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:2479883/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:434657/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:3015307/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:2636397/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:4565468/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:2483040/1‑71 (MQ=255)                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                >  1:1988423/1‑71 (MQ=255)                                aTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                <  1:1878548/70‑1 (MQ=255)                                aTTCTTGCCTCCAGGGCGCGGTACCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                <  1:1143936/70‑1 (MQ=255)                                  tCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                             >  1:481474/1‑71 (MQ=255)                                   cTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGtt                            >  1:4335050/1‑71 (MQ=255)                                   cTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                             >  1:3512693/1‑70 (MQ=255)                                   cTTGCCTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCATTATTAGc                                           >  1:3355906/1‑56 (MQ=255)                                   cTTGCCTCCAGGGCGCGCTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                             <  1:3157757/70‑1 (MQ=255)                                        cTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCccttcct                                                  <  1:3564317/44‑1 (MQ=255)                                        cTCCAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCccttcct                                                  <  1:710037/44‑1 (MQ=255)                                          ccAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCtta                                                >  1:4333593/1‑44 (MQ=255)                                           cAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                     >  1:950687/1‑70 (MQ=255)                                           cAGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                     >  1:1996582/1‑70 (MQ=255)                                            aGGGCGCGGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACa                   <  1:3499670/71‑1 (MQ=255)                                             gggCGCGTTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaa                       >  1:1532486/1‑66 (MQ=255)                                                 gcgGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc              >  1:2603350/1‑71 (MQ=255)                                                 gcgGTAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc              <  1:2469861/71‑1 (MQ=255)                                                     tAGCCGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                <  1:3547818/65‑1 (MQ=255)                                                         cGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt       >  1:2674635/1‑70 (MQ=255)                                                         cGCTGCGCTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt       <  1:4297819/70‑1 (MQ=255)                                                          gCTGCGCTCTGTCAATTTCCCTTACTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTa     <  1:2193097/71‑1 (MQ=255)                                                             gcgcTCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGt  >  1:723224/1‑71 (MQ=255)                                                                 |                                                                   ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT  >  W3110S.gb/4615654‑4615784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑