Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	556,858	A→T	L36Q (CTG→CAG)	folD ←	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	556,858	0	A	T	100.0%	118.6 / NA	40	L36Q (CTG→CAG)	folD	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/19);  total (21/19)

CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCT  >  W3110S.gb/556790‑556924                                                                     |                                                                   cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt                                                                  >  1:3747331/1‑71 (MQ=255) cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt                                                                  >  1:3106131/1‑71 (MQ=255)  acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc                                                                 <  1:31010/71‑1 (MQ=255)   caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc                                                                <  1:1731780/71‑1 (MQ=255)   caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc                                                                <  1:3375021/71‑1 (MQ=255)   caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc                                                                <  1:2379297/71‑1 (MQ=255)    aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt                                                               <  1:4197718/71‑1 (MQ=255)       gcTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACCGCCTGTCCt                                                               <  1:4608611/67‑1 (MQ=255)           gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                              >  1:766923/1‑65 (MQ=255)                tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt                                                   >  1:1236953/1‑71 (MQ=255)                tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt                                                   >  1:761572/1‑71 (MQ=255)                          aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa                                         <  1:939995/71‑1 (MQ=255)                          aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa                                         <  1:1739027/71‑1 (MQ=255)                           tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc                                                                <  1:2042930/47‑1 (MQ=255)                           tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                           <  1:1795734/68‑1 (MQ=255)                             aaTTTGCGATGCAGGGTTACTACCCACCAGCAGAACGGCCTGTCCTg                                                              >  1:1462501/1‑47 (MQ=255)                             aaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                              >  1:4284883/1‑47 (MQ=255)                             aaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                                       <  1:2029613/70‑1 (MQ=255)                             aaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                                       <  1:1548922/70‑1 (MQ=255)                                        cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                                       >  1:1707182/1‑59 (MQ=255)                                        cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt                           >  1:1528206/1‑71 (MQ=255)                                             ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt                       >  1:609381/1‑70 (MQ=255)                                             ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt                       >  1:3087498/1‑70 (MQ=255)                                                   cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa                  <  1:2158051/69‑1 (MQ=255)                                                   cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc                >  1:3113463/1‑71 (MQ=255)                                                   cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc                >  1:432348/1‑71 (MQ=255)                                                   cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc                >  1:2328477/1‑71 (MQ=255)                                                      accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc             <  1:2289077/71‑1 (MQ=255)                                                        caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCag           <  1:4063045/71‑1 (MQ=255)                                                        caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCag           <  1:4443160/71‑1 (MQ=255)                                                         aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa            >  1:3253722/1‑69 (MQ=255)                                                         aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa            >  1:1734707/1‑69 (MQ=255)                                                         aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa            >  1:2739327/1‑69 (MQ=255)                                                         aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa            >  1:2274035/1‑69 (MQ=255)                                                          gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCagag         <  1:3620196/71‑1 (MQ=255)                                                            acaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg       >  1:3357133/1‑71 (MQ=255)                                                            acaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg       >  1:2542855/1‑71 (MQ=255)                                                            acaACGGCCTGTCATGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg       >  1:3802214/1‑71 (MQ=255)                                                             caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc      <  1:197024/71‑1 (MQ=255)                                                                 ggCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACct  <  1:1134277/71‑1 (MQ=255)                                                                     |                                                                   CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCT  >  W3110S.gb/556790‑556924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑