| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 630,265 | T→C | C383C (TGT→TGC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 630,265 | 0 | T | C | 98.0% | 169.1 / ‑4.6 | 51 | C383C (TGT→TGC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/25); minor base G (1/0); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGG > W3110S.gb/630198‑630327 | gCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCat > 1:4092012/1‑70 (MQ=255) cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCatca > 1:3827677/1‑71 (MQ=255) cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCatca > 1:3160188/1‑71 (MQ=255) cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCatca > 1:2264064/1‑71 (MQ=255) ttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCatcatc > 1:4415050/1‑71 (MQ=255) tACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCggg > 1:1312028/1‑71 (MQ=255) tACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCggg > 1:2136511/1‑71 (MQ=255) gTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCgtg < 1:3332086/71‑1 (MQ=255) gTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCgtg < 1:2707558/71‑1 (MQ=255) ggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTa < 1:4473475/70‑1 (MQ=255) tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTAttt > 1:1900714/1‑70 (MQ=255) tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCAGGGCGTGTAtt > 1:31/1‑69 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGc > 1:1150615/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGc > 1:3607811/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGc > 1:2024816/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGc > 1:1261737/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTAATTTg > 1:1474774/1‑70 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTAttt > 1:2802974/1‑66 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATg < 1:4326785/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATg < 1:2707093/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3046771/71‑1 (MQ=255) ggAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATg < 1:798884/70‑1 (MQ=255) gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGa > 1:2080170/1‑70 (MQ=255) aaTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGa < 1:2101074/46‑1 (MQ=255) aaTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGa < 1:2665176/46‑1 (MQ=255) aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTAttt > 1:1207193/1‑60 (MQ=255) aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTTCCATGaa > 1:3420877/1‑69 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTa < 1:155179/56‑1 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTa < 1:1468678/56‑1 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3457725/66‑1 (MQ=255) cTTCGTGGCGATTATGGCTCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc > 1:1788513/1‑71 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTTCCTCCTGCATCATCGATCCGGGCGTGTATTTTTcc > 1:2828668/1‑61 (MQ=25) cGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1093948/69‑1 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATAGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1883934/69‑1 (MQ=255) gTGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGAt < 1:2153917/71‑1 (MQ=255) tGGCGATTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGc < 1:3425648/55‑1 (MQ=255) gATTATGGCGCTGGTTTCCGCCTGCATCATAGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCgg < 1:1549784/71‑1 (MQ=255) aTTATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCgg < 1:813524/70‑1 (MQ=255) ttATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGaa < 1:2871852/55‑1 (MQ=255) tATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTAttt > 1:3554165/1‑45 (MQ=255) tATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGcc > 1:2842794/1‑49 (MQ=255) tATGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGc > 1:227373/1‑71 (MQ=255) tGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCt < 1:4595918/70‑1 (MQ=255) tGGCGCTGGTTTCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTg < 1:1977126/71‑1 (MQ=255) tCCGCCTGGATCATCGATCCGGGCGTGTATTTTGCCa > 1:4255184/1‑37 (MQ=255) tCCGCCTGCATCATCGATCCGGGGGTGTATTTTGCCa > 1:3647908/1‑37 (MQ=38) tCCGCCTGCATCATCGATCCGGGCGTGTATTTTGCCa > 1:2122313/1‑37 (MQ=255) ccGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAgg < 1:2247330/70‑1 (MQ=255) ccGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAgg < 1:246059/70‑1 (MQ=255) ccGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAgg < 1:90984/70‑1 (MQ=255) ccGCCTGCATCATCGATCCGGGCGTGTATTTTGCCATGAACACCCCGATGGCGGTGCTGGCTCCGGCAgg < 1:4618335/70‑1 (MQ=255) | GCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGG > W3110S.gb/630198‑630327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |