Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	121,347	T→C	M69V (ATG→GTG)	aroP ←	aromatic amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	121,347	0	T	C	100.0%	194.1 / NA	56	M69V (ATG→GTG)	aroP	aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

CAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCT  >  W3110S.gb/121292‑121414                                                        |                                                                    cAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACg                                                      <  1:4349966/71‑1 (MQ=255)    tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                                   >  1:1433360/1‑71 (MQ=255)     aTTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCActt                                                                   >  1:1248225/1‑54 (MQ=255)     aTTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                                   >  1:3986092/1‑70 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCTTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:3435935/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCCCCACTTCACCCAGCTGACGCATGa                                                 >  1:2560515/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                                  <  1:2478417/70‑1 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                                  <  1:2472859/70‑1 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:831841/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:4189028/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:3792673/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:621867/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:558996/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:131318/1‑71 (MQ=255)      tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa                                                 >  1:1339729/1‑71 (MQ=255)        tataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCAt                                                   >  1:2504026/1‑67 (MQ=255)        tataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGAt                                                >  1:3552074/1‑70 (MQ=255)         ataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGAt                                                >  1:4576528/1‑69 (MQ=255)         ataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGAt                                                >  1:3846118/1‑69 (MQ=255)            aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGa                                            <  1:2774237/70‑1 (MQ=255)            aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGa                                            <  1:3039998/70‑1 (MQ=255)              cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGa                                                        >  1:3698258/1‑56 (MQ=255)                  gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                                     >  1:4580213/1‑71 (MQ=255)                   tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGAt                                    <  1:419706/71‑1 (MQ=255)                    ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGAt                                    >  1:386818/1‑70 (MQ=255)                     gCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaa                                  <  1:405638/71‑1 (MQ=255)                     gCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaa                                  <  1:299677/71‑1 (MQ=255)                      cTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaa                                 <  1:4378074/71‑1 (MQ=255)                        aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaaa                                >  1:603089/1‑70 (MQ=255)                        aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc                               >  1:4488486/1‑71 (MQ=255)                        aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc                               >  1:2857425/1‑71 (MQ=255)                        aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc                               >  1:2029518/1‑71 (MQ=255)                        aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc                               >  1:2113348/1‑71 (MQ=255)                            gAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:4673220/71‑1 (MQ=255)                            gAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:1190802/71‑1 (MQ=255)                            gAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:4594515/71‑1 (MQ=255)                            gAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:2860750/71‑1 (MQ=255)                              gCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa                         >  1:120567/1‑71 (MQ=255)                               ccTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAAt                        <  1:3840250/71‑1 (MQ=255)                                          ttcttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAcc               >  1:4520704/1‑69 (MQ=255)                                           tcttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATgg                      >  1:3541333/1‑61 (MQ=255)                                           tcttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg            >  1:2071428/1‑71 (MQ=255)                                           tcttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg            >  1:1903358/1‑71 (MQ=255)                                            cttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa                         >  1:1470034/1‑57 (MQ=255)                                            cttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg            <  1:509595/70‑1 (MQ=255)                                             ttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg            <  1:1163159/69‑1 (MQ=255)                                               cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt         >  1:971339/1‑70 (MQ=255)                                               cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt         <  1:4378078/70‑1 (MQ=255)                                               cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt         <  1:2156810/70‑1 (MQ=255)                                               cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt         <  1:1514584/70‑1 (MQ=255)                                                 accaccACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAAt      <  1:4194515/71‑1 (MQ=255)                                                 accaccACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAAt      <  1:927672/71‑1 (MQ=255)                                                 accaccACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAAt      <  1:4281196/71‑1 (MQ=255)                                                  ccaccaCTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATc     <  1:2371147/71‑1 (MQ=255)                                                    accaCTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:4511448/47‑1 (MQ=255)                                                    accaCTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc                           <  1:3787967/47‑1 (MQ=255)                                                     ccaCTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCt  <  1:3558387/71‑1 (MQ=255)                                                        |                                                                    CAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCT  >  W3110S.gb/121292‑121414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑