Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	746,293	C→T	A236V (GCC→GTC)	ybgL →	predicted lactam utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	746,293	0	C	T	100.0%	124.7 / NA	43	A236V (GCC→GTC)	ybgL	predicted lactam utilization protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/27);  total (16/27)

CGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAT  >  W3110S.gb/746227‑746358                                                                   |                                                                  cGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGaa                                                               <  1:224883/71‑1 (MQ=255)        cctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGa                                                        >  1:806714/1‑71 (MQ=255)             aTGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGAttgtt                                                   <  1:4037975/71‑1 (MQ=255)                gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc                                                >  1:3952794/1‑71 (MQ=255)                gCGACGGCCAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc                                                >  1:4401427/1‑71 (MQ=255)                                 cTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGAtt                               <  1:3169078/71‑1 (MQ=255)                                   ggCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGaaaa                                                             >  1:1683117/1‑39 (MQ=255)                                         cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg                        >  1:4356421/1‑70 (MQ=255)                                         cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg                        >  1:3519595/1‑70 (MQ=255)                                                 gACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGaaaaa               <  1:2855813/71‑1 (MQ=255)                                                   cTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             <  1:184930/71‑1 (MQ=255)                                                   cTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             <  1:2064455/71‑1 (MQ=255)                                                    tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAAccc                                    >  1:3767295/1‑47 (MQ=255)                                                    tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             >  1:980373/1‑70 (MQ=255)                                                    tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             >  1:3550713/1‑70 (MQ=255)                                                    tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             >  1:102121/1‑70 (MQ=255)                                                    tACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             >  1:4591794/1‑70 (MQ=255)                                                    tACGCTCTGCATTT‑TCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGa           >  1:3950697/1‑71 (MQ=255)                                                      cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAgg            <  1:3874755/69‑1 (MQ=255)                                                      cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg             <  1:2297908/68‑1 (MQ=255)                                                      cGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGa           >  1:2890958/1‑70 (MQ=255)                                                        ctctGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGatat        <  1:339924/71‑1 (MQ=255)                                                          ctGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCa      >  1:3548556/1‑71 (MQ=255)                                                           tGCATTTGTCGAAAAGGGGATTGTTGTCTCAGCAt                                         <  1:771683/35‑1 (MQ=37)                                                           tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt                                         <  1:3739765/35‑1 (MQ=255)                                                           tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt                                         <  1:136101/35‑1 (MQ=255)                                                           tGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCAt                                         <  1:666098/35‑1 (MQ=255)                                                            gCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATa                                        >  1:4400247/1‑35 (MQ=255)                                                            gCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAAc                                      >  1:1536708/1‑37 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:11079/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:3653255/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:54512/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:540203/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:4530905/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:1681550/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:2091310/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:2092420/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:2406056/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:241829/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:2813594/69‑1 (MQ=255)                                                             cATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:310886/69‑1 (MQ=255)                                                              aTTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt  <  1:4006185/71‑1 (MQ=255)                                                               tttGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc     <  1:640674/67‑1 (MQ=255)                                                                   |                                                                  CGGTCTGCCTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAT  >  W3110S.gb/746227‑746358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑