Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	847,104	A→G	R146R (CGT→CGC)	glnP ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	847,104	0	A	G	100.0%	86.2 / NA	26	R146R (CGT→CGC)	glnP	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

TCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGT  >  W3110S.gb/847040‑847170                                                                 |                                                                   tCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGc                                                              <  1:1349450/71‑1 (MQ=255) tCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGc                                                              <  1:3788050/71‑1 (MQ=255)  cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcgacg                                                               >  1:1153862/1‑69 (MQ=255)  cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcgacg                                                               >  1:1829238/1‑69 (MQ=255)  cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcgacg                                                               >  1:3731723/1‑69 (MQ=255)  cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcgacg                                                               >  1:730729/1‑69 (MQ=255)  cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGc                                                              <  1:4548236/70‑1 (MQ=255)               gAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTaa                                                <  1:1458913/71‑1 (MQ=255)                  gtgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAAt                                             <  1:653957/71‑1 (MQ=255)                    gtCTTTAATGCTGATGATCTACTGGTTACCCAGCGGCGGCAGCATGCGACGCAg                                                            >  1:1058226/1‑54 (MQ=255)                    gtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcga                                                                 >  1:2468864/1‑49 (MQ=255)                    gtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGa                                           >  1:3667564/1‑71 (MQ=255)                     tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAg                                                            >  1:2178949/1‑53 (MQ=255)                     tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAg                                                            >  1:2173734/1‑53 (MQ=255)                     tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAAt                                             >  1:841301/1‑68 (MQ=255)                     tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGAc                                          >  1:1350354/1‑71 (MQ=255)                     tCTTTAACGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGAc                                          >  1:3059199/1‑71 (MQ=255)                            tGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGa                                   <  1:1106756/71‑1 (MQ=255)                                 atgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa                                  >  1:513386/1‑67 (MQ=255)                                 atgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGt                              <  1:2151887/71‑1 (MQ=255)                                 atgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTCCCAGCGGTAAAATGACGTAGCGAATGGt                              <  1:2268005/71‑1 (MQ=255)                                  tgatCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCAAGCGGTAAAATGACGTAGCGAATGGtt                             >  1:987119/1‑71 (MQ=255)                                       cACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTccc                         <  1:2595750/70‑1 (MQ=255)                                           ggTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGt                    >  1:2259958/1‑71 (MQ=255)                                             ttACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa                  <  1:3072580/71‑1 (MQ=255)                                             ttACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa                  <  1:2030544/71‑1 (MQ=255)                                                cccAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGaaa                <  1:541396/70‑1 (MQ=255)                                                          gcagcaTGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGcc     <  1:3996873/71‑1 (MQ=255)                                                            agcaTGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAACGAGCGCCAg   >  1:1546311/1‑71 (MQ=255)                                                             gcaTGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGt  <  1:4173126/71‑1 (MQ=255)                                                             gcaTGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGt  <  1:4662280/71‑1 (MQ=255)                                                                 |                                                                   TCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGT  >  W3110S.gb/847040‑847170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑