| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 887,947 | G→A | A35T (GCT→ACT) | ybjK → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 887,947 | 0 | G | A | 97.8% | 128.0 / ‑5.7 | 45 | A35T (GCT→ACT) | ybjK | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/20); minor base C (0/1); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACT > W3110S.gb/887882‑888014 | ttATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTAcc < 1:1212419/71‑1 (MQ=255) gCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCgg > 1:4562765/1‑71 (MQ=255) cACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCgggg > 1:1157175/1‑71 (MQ=255) cACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCgggg > 1:1952529/1‑71 (MQ=255) gCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTggg < 1:4633339/71‑1 (MQ=255) gCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTggg < 1:19703/71‑1 (MQ=255) tGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTAcc < 1:2478501/47‑1 (MQ=255) gAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGa > 1:819402/1‑71 (MQ=255) gAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGa > 1:1515817/1‑71 (MQ=255) gAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGa > 1:2240984/1‑71 (MQ=255) gAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGa > 1:3947992/1‑71 (MQ=255) gAAACTTTACGGAATACATGCTGTTACGCAACGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGa > 1:573689/1‑71 (MQ=255) aaCTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATg < 1:828309/71‑1 (MQ=255) aaCTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATg < 1:4100161/71‑1 (MQ=255) aaCTTTACGGAATACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATg < 1:4008538/71‑1 (MQ=255) tACATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATc < 1:45230/55‑1 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttttt > 1:2802230/1‑70 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttttt > 1:4485465/1‑70 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttttt > 1:1714651/1‑70 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttttt > 1:569754/1‑70 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttttt > 1:3173818/1‑70 (MQ=255) cATGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAtttt > 1:324546/1‑69 (MQ=255) tGCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAg > 1:4532164/1‑71 (MQ=255) gCTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAg > 1:658262/1‑70 (MQ=255) cTGTTACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAgg > 1:678939/1‑70 (MQ=255) ttATGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAtt < 1:2989748/71‑1 (MQ=255) ttACGCACCGCAAAATTCCTACCCTTGCCGGTGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAtt < 1:3029511/71‑1 (MQ=255) tACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAtt > 1:304045/1‑70 (MQ=255) tACGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAtt > 1:4591654/1‑70 (MQ=255) aCGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGaa > 1:2155409/1‑67 (MQ=255) aCGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAtt < 1:1503306/69‑1 (MQ=255) aCGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtga > 1:3908944/1‑71 (MQ=255) aCGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtg < 1:4258456/70‑1 (MQ=255) cGCACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtgat < 1:695956/71‑1 (MQ=255) cACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtgatga < 1:1223116/71‑1 (MQ=255) cACCGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtgatga < 1:2358902/71‑1 (MQ=255) ccGCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCg < 1:1403801/40‑1 (MQ=255) gCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttg > 1:4164591/1‑71 (MQ=255) gCAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttg > 1:2248564/1‑71 (MQ=255) cAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttgt < 1:2112958/71‑1 (MQ=255) cAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttgt < 1:3939501/71‑1 (MQ=255) cAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttgt < 1:3062470/71‑1 (MQ=255) cAAAATTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttgt < 1:2453061/71‑1 (MQ=255) aaaaTTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAgttgt < 1:3443336/70‑1 (MQ=255) aTTACTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACt > 1:3420365/1‑71 (MQ=255) | TTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACT > W3110S.gb/887882‑888014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |