Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	915,474	G→A	intergenic (‑195/+300)	ybjE ← / ← aqpZ	predicted transporter/aquaporin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	915,474	0	G	A	100.0%	84.4 / NA	29	intergenic (‑195/+300)	ybjE/aqpZ	predicted transporter/aquaporin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/21);  total (8/21)

ATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGG  >  W3110S.gb/915406‑915530                                                                     |                                                         aTTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCAtt                                                        <  1:3632764/71‑1 (MQ=255) aTTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCAtt                                                        <  1:3188684/71‑1 (MQ=255)  tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATTTTGGTGGCGTCAAAATCGCCGAGGCAtt                                                        <  1:3151506/70‑1 (MQ=255)  tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTc                                                       <  1:2417626/71‑1 (MQ=255)  tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTc                                                       <  1:3583556/71‑1 (MQ=255)  tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTc                                                       <  1:4195026/71‑1 (MQ=255)    tAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTccc                                                     >  1:2820369/1‑71 (MQ=255)    tAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTc                                                       >  1:1945475/1‑69 (MQ=255)      aaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTccc                                                     <  1:1961225/69‑1 (MQ=255)      aaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTg                                                   <  1:4635030/71‑1 (MQ=255)      aaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTg                                                   <  1:4242380/71‑1 (MQ=255)      aaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTg                                                   <  1:3755769/71‑1 (MQ=255)      aaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTg                                                   <  1:3349347/71‑1 (MQ=255)           atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGc                                              >  1:3124384/1‑71 (MQ=255)           atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGc                                              >  1:4647955/1‑71 (MQ=255)           atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGc                                              >  1:1259487/1‑71 (MQ=255)            ttCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGcc                                             >  1:1274456/1‑71 (MQ=255)              cTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCAtt                                                        >  1:1983175/1‑58 (MQ=255)                  cgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGc                                        <  1:1260252/70‑1 (MQ=255)                   gcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAg                                      <  1:2399191/71‑1 (MQ=255)                        aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCca                                  <  1:2920609/70‑1 (MQ=255)                        aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCca                                  <  1:23424/70‑1 (MQ=255)                        aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCca                                  <  1:1267571/70‑1 (MQ=255)                        aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCca                                  <  1:727941/70‑1 (MQ=255)                             cAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCCACATGGa                            <  1:3204968/71‑1 (MQ=255)                             cAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCCACATGGa                            <  1:4540281/71‑1 (MQ=255)                                    cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCCACATGGATGTggg                      <  1:3905601/70‑1 (MQ=255)                                     aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCt                    <  1:2427870/71‑1 (MQ=255)                                                       aaaaTCGCCGAGGCATTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAgg  >  1:4319148/1‑71 (MQ=21)                                                                     |                                                         ATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGG  >  W3110S.gb/915406‑915530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑