Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,013,795	A→G	S39G (AGC→GGC)	pqiB →	paraquat‑inducible protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,013,795	0	A	G	100.0%	149.0 / NA	43	S39G (AGC→GGC)	pqiB	paraquat‑inducible protein B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/27);  total (16/27)

GTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAACC  >  W3110S.gb/1013728‑1013860                                                                    |                                                                  gTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGc                                                                 >  1:2035225/1‑70 (MQ=255)      ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCAt                                                              <  1:2588986/68‑1 (MQ=255)      ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAg                                                           <  1:1472632/71‑1 (MQ=255)      ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAg                                                           >  1:2913395/1‑71 (MQ=255)       cGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGATTCTTTTTTATCATTACGGCCATCAgg                                                          <  1:2810755/71‑1 (MQ=255)          gtgGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGa                                                        >  1:2802217/1‑70 (MQ=255)             gATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCa                                                            >  1:16829/1‑63 (MQ=255)              atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa                                                   <  1:601446/71‑1 (MQ=255)              atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa                                                   <  1:3807962/71‑1 (MQ=255)              atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa                                                   <  1:3262985/71‑1 (MQ=255)              atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa                                                   <  1:2895327/71‑1 (MQ=255)                   tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGtt                                               >  1:3364288/1‑69 (MQ=255)                   tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGa                                              >  1:1127452/1‑71 (MQ=255)                   tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGa                                              >  1:4219394/1‑71 (MQ=255)                        tCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTg                                         <  1:3418492/71‑1 (MQ=255)                          gTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGaa                                                  >  1:3234524/1‑60 (MQ=255)                          gTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGAt                                       >  1:2162972/1‑71 (MQ=255)                                gcgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCa                                     >  1:550400/1‑67 (MQ=255)                                 cgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:3911686/71‑1 (MQ=255)                                 cgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:1479598/71‑1 (MQ=255)                                  gcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:3512568/70‑1 (MQ=255)                                    tCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCAt                                                              <  1:4304679/38‑1 (MQ=255)                                         ggggCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCgg                         >  1:3524881/1‑70 (MQ=255)                                              cTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagg                     <  1:3303291/69‑1 (MQ=255)                                              cTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagg                     <  1:2939022/69‑1 (MQ=255)                                               tGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGAAAGTGACCCt                                          <  1:2713954/47‑1 (MQ=38)                                                 ggTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTg                <  1:2188591/71‑1 (MQ=255)                                                  gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:3779661/54‑1 (MQ=255)                                                  gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:3920851/54‑1 (MQ=255)                                                  gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg                                <  1:2754328/54‑1 (MQ=255)                                                  gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagga                    <  1:1798933/66‑1 (MQ=255)                                                  gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagga                    <  1:1158679/66‑1 (MQ=255)                                                   ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTg                >  1:2545730/1‑69 (MQ=255)                                                   ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGaa              <  1:1025445/71‑1 (MQ=255)                                                   ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGaa              <  1:2880746/71‑1 (MQ=255)                                                     cTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGaa                              >  1:191178/1‑53 (MQ=255)                                                     cTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGCAATTGAAgg            >  1:4500018/1‑71 (MQ=255)                                                        ttttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGa               >  1:3160840/1‑65 (MQ=255)                                                        ttttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAgg            <  1:4335292/68‑1 (MQ=255)                                                         tttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTgg         >  1:2897568/1‑70 (MQ=255)                                                             tCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCaa      <  1:2924727/69‑1 (MQ=255)                                                              cATTACGGCGATCAGGGACCGGAAGTGACCCTGATGACCGCGAATGCGGAAGGAATTGaa              <  1:1478972/60‑1 (MQ=255)                                                               aTTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAacc  <  1:1414372/71‑1 (MQ=255)                                                                    |                                                                  GTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAACC  >  W3110S.gb/1013728‑1013860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑