Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,057,926	G→A	R151W (CGG→TGG)	torR ←	DNA‑binding response regulator in two‑component regulatory system with TorS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,057,926	0	G	A	100.0%	135.3 / NA	45	R151W (CGG→TGG)	torR	DNA‑binding response regulator in two‑component regulatory system with TorS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/30);  total (15/30)

CGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCGCTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGA  >  W3110S.gb/1057858‑1057987                                                                     |                                                              cGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACt                                                             >  1:1060777/1‑71 (MQ=255)     ttCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAg                                                         >  1:881375/1‑71 (MQ=255)       cGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       >  1:1618208/1‑71 (MQ=255)       cGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       >  1:2490720/1‑71 (MQ=255)        gTCACAAATGCCACCAACATTTCATACTCTGCGCTGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:2261180/70‑1 (MQ=255)        gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:1242320/70‑1 (MQ=255)        gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:847329/70‑1 (MQ=255)        gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:1658383/70‑1 (MQ=255)        gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:3015046/70‑1 (MQ=255)              aaTGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGgcg                                                >  1:4074413/1‑71 (MQ=255)                     ccaACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:3002119/57‑1 (MQ=255)                       aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa                                                     >  1:330548/1‑57 (MQ=255)                       aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa                                                     >  1:2514920/1‑57 (MQ=255)                       aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa                                                     >  1:3061964/1‑57 (MQ=255)                       aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACAtt                                       >  1:2164794/1‑71 (MQ=255)                         cATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACAtt                                       >  1:1100729/1‑69 (MQ=255)                          aTTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAg                                    >  1:293705/1‑71 (MQ=255)                             tCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc                                  <  1:244080/70‑1 (MQ=255)                             tCATACTCTGCGCGGGTCAGTTTAATCGGCGCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc                                  <  1:285109/70‑1 (MQ=255)                               aTACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAAt                               <  1:2638140/71‑1 (MQ=255)                                tACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATa                              <  1:2349213/71‑1 (MQ=255)                                  ctctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATaa                             <  1:533304/70‑1 (MQ=255)                                   tctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc                                  <  1:384075/64‑1 (MQ=255)                                   tctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAAcc                           >  1:1691027/1‑71 (MQ=255)                                   tctGCGCGGGGCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc                                  <  1:554858/64‑1 (MQ=39)                                     tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCaa                                >  1:842915/1‑64 (MQ=255)                                     tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCgg                         >  1:173257/1‑71 (MQ=255)                                     tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCgg                         >  1:4221204/1‑71 (MQ=255)                                           ggTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg                                                       <  1:3217220/35‑1 (MQ=255)                                           ggTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGc                   <  1:179666/71‑1 (MQ=255)                                            gTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGc                   <  1:4593901/70‑1 (MQ=255)                                            gTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCg                  <  1:4049125/71‑1 (MQ=255)                                             tCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGa                 <  1:3381062/71‑1 (MQ=255)                                             tCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGa                 <  1:3922308/71‑1 (MQ=255)                                                 tttAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGc             <  1:1586874/71‑1 (MQ=255)                                                        ggCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGt       <  1:3186127/70‑1 (MQ=255)                                                        ggCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGt       <  1:2056027/70‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCTGCAAAGCGATAGCAGTTGTCCTg   <  1:40680/69‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg   <  1:4592674/69‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg   <  1:4624562/69‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg   <  1:2935088/69‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg   <  1:2287037/69‑1 (MQ=255)                                                             gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGa  <  1:4351671/70‑1 (MQ=255)                                                              ccATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg   <  1:1732897/68‑1 (MQ=255)                                                                 tCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATa                              <  1:4278171/38‑1 (MQ=255)                                                                     |                                                              CGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCGCTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGA  >  W3110S.gb/1057858‑1057987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑