Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,068,568	T→A	G22G (GGT→GGA)	ymdF →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,068,568	0	T	A	100.0%	105.1 / NA	36	G22G (GGT→GGA)	ymdF	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/13);  total (23/13)

AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAG  >  W3110S.gb/1068502‑1068629                                                                   |                                                              aaTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaa                                                            >  1:676362/1‑70 (MQ=255)  aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa                                                          <  1:3361608/71‑1 (MQ=255)  aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaa                                                           >  1:1114940/1‑70 (MQ=255)   tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa                                                         <  1:1910056/71‑1 (MQ=255)   tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa                                                         <  1:1100890/71‑1 (MQ=255)     gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:944184/1‑71 (MQ=255)     gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:4571207/1‑71 (MQ=255)     gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:1832254/1‑71 (MQ=255)     gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:1946649/1‑71 (MQ=255)        aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg                                                     <  1:2813771/70‑1 (MQ=255)        aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg                                                     <  1:2804352/70‑1 (MQ=255)          ccATCGAGGCGGTTCCGGCAATTTTGCAGAAGACAGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGa                                                   >  1:2637177/1‑70 (MQ=255)               gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:3174507/1‑61 (MQ=255)               gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg                                                       >  1:4487901/1‑61 (MQ=255)               gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacagca                                              >  1:4480529/1‑70 (MQ=255)               gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGa                                                   >  1:705419/1‑65 (MQ=255)                       tCCGGCAATTTTGCAGAAGCCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg                                     >  1:2201901/1‑71 (MQ=39)                       tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg                                     >  1:1754822/1‑71 (MQ=255)                       tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg                                     >  1:3838124/1‑71 (MQ=255)                       accGGCAATTTTGCAGAAGCCCGCGAAAGAGCATCAGAAGCAGGAAAAAAATGTGGACAGCACAGCggggg                                     >  1:3160096/2‑71 (MQ=255)                        ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggt                                     >  1:4450420/1‑69 (MQ=255)                        ccGGCAATTTTGCAGAAGACAGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggt                                     >  1:1459272/1‑69 (MQ=255)                         cGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTa                                   <  1:2026584/71‑1 (MQ=255)                         cGGCAATTTTGAAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTa                                   <  1:4350992/71‑1 (MQ=255)                                tttGGAGAAGACCGAGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg                                                     <  1:3953824/46‑1 (MQ=37)                                  tGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg                           <  1:4112040/70‑1 (MQ=255)                                   gCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGa                          <  1:4682968/70‑1 (MQ=255)                                     agaagaCCGCGAAAGAGCATCAGAATCAGGAAAAAAAGGTGGACAGCACAGCGGTGGTaa                                  <  1:944984/60‑1 (MQ=38)                                     agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTaa                                  <  1:825640/60‑1 (MQ=255)                                      gaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGa                      <  1:684940/71‑1 (MQ=255)                                           ccGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAttt                               >  1:1942290/1‑57 (MQ=255)                                           ccGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAttt                               >  1:1346200/1‑57 (MQ=255)                                                  agagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGAcc                    >  1:3917567/1‑61 (MQ=255)                                                  agagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGAcc                    >  1:3101335/1‑61 (MQ=255)                                                   gagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa         >  1:1257390/1‑71 (MQ=255)                                                    agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt        >  1:3357109/1‑71 (MQ=255)                                                     gCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgcgcgc          <  1:942065/68‑1 (MQ=255)                                                          aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAg  >  1:701938/1‑71 (MQ=255)                                                                   |                                                              AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAG  >  W3110S.gb/1068502‑1068629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑