Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,686	0	T	C	100.0%	154.1 / NA	45	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/14);  total (31/14)

ATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTT  >  W3110S.gb/1093626‑1093749                                                             |                                                                aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGaa                                                        >  1:1265928/1‑70 (MQ=255)  tGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa                                                      <  1:4048404/71‑1 (MQ=255)       ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                 <  1:969074/71‑1 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:4058383/1‑71 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:3122991/1‑71 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:105055/1‑71 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:3378990/1‑71 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:598267/1‑71 (MQ=255)         gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                               >  1:2169159/1‑71 (MQ=255)             tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt                                                              <  1:3227909/52‑1 (MQ=255)              cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                           >  1:1601255/1‑70 (MQ=255)               tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAt                                                               >  1:2211158/1‑49 (MQ=255)               tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa                                         >  1:1244057/1‑71 (MQ=255)                 ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                           <  1:708262/67‑1 (MQ=255)                 ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                           <  1:212156/67‑1 (MQ=255)                 ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                           <  1:723471/67‑1 (MQ=255)                 ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                           <  1:2896200/67‑1 (MQ=255)                  tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca                                       >  1:1255920/1‑70 (MQ=255)                   attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg                                                   >  1:2163098/1‑57 (MQ=255)                   attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg                                                   >  1:521366/1‑57 (MQ=255)                    tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                    <  1:3476874/71‑1 (MQ=255)                     ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                    <  1:1636850/70‑1 (MQ=255)                               aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat                         >  1:410127/1‑71 (MQ=255)                               aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat                         >  1:415828/1‑71 (MQ=255)                                 aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                       <  1:2694266/71‑1 (MQ=255)                                  tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                      >  1:936514/1‑71 (MQ=255)                                        tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTc                 >  1:127088/1‑70 (MQ=255)                                             atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa           <  1:2822959/71‑1 (MQ=255)                                             atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa           <  1:62046/71‑1 (MQ=255)                                                ttAATATTTCTGGCATTTGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:1691438/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa                                         >  1:3073196/1‑38 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:3317936/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:3038821/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:546675/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:2151027/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:2145161/1‑71 (MQ=255)                                                ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt        >  1:2041284/1‑71 (MQ=255)                                                ttAATAATTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCa                           >  1:4466385/1‑52 (MQ=255)                                                  aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGa      >  1:1125296/1‑71 (MQ=255)                                                    tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGAca    <  1:4222500/71‑2 (MQ=255)                                                    tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGAca    <  1:168108/71‑2 (MQ=255)                                                     atTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACtt   >  1:2728606/1‑71 (MQ=255)                                                      tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCaa                          >  1:3896975/1‑47 (MQ=255)                                                      tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACttt  >  1:4053497/1‑71 (MQ=255)                                                      tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACttt  >  1:3242178/1‑71 (MQ=255)                                                             |                                                                ATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTT  >  W3110S.gb/1093626‑1093749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑