Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,101,111 G→A S272L (TCG→TTG)  csgG ← outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,101,1110GA100.0% 116.6 / NA 39S272L (TCG→TTG) csgGouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/29);  total (10/29)

CGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAC  >  W3110S.gb/1101045‑1101177
                                                                  |                                                                  
cGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa                                                                 <  1:3656387/70‑1 (MQ=255)
       aCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCgg                                                         <  1:1235554/71‑1 (MQ=255)
       aCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCgg                                                         <  1:2221393/71‑1 (MQ=255)
         aGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTa                                                       <  1:565554/71‑1 (MQ=255)
          gTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa                                                                 <  1:2747273/60‑1 (MQ=255)
          gTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAt                                                      <  1:1684267/71‑1 (MQ=255)
             aaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAttt                                                    >  1:571700/1‑70 (MQ=255)
                tGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACata                                                               >  1:520507/1‑56 (MQ=255)
                  ccGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCgg                                                         <  1:2216309/60‑1 (MQ=255)
                  ccGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAg                                              <  1:1493727/71‑1 (MQ=255)
                     gatgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAAt                                           <  1:672966/71‑1 (MQ=255)
                       tgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCgg                                                         <  1:2001979/55‑1 (MQ=255)
                       tgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGt                                         <  1:2920575/71‑1 (MQ=255)
                       tgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGt                                         <  1:3859757/71‑1 (MQ=255)
                          tAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAttt                                                    <  1:4267581/57‑1 (MQ=255)
                          tAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAttt                                                    <  1:1353177/57‑1 (MQ=255)
                          tAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCAt                                      <  1:2220065/71‑1 (MQ=255)
                          tAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCAt                                      <  1:3715873/71‑1 (MQ=255)
                            aTTCCGGCTTTTTTATCTGTCAGGTTTCCGGTGGAACCAACATATGTCGGTATTTCACCAg                                              <  1:3577586/61‑1 (MQ=255)
                               ccGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAt                                                      >  1:3009788/1‑50 (MQ=255)
                                  gCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGt                              <  1:3987191/71‑1 (MQ=255)
                                   cTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAg                                              <  1:4448585/54‑1 (MQ=255)
                                    ttttttATCTGTCAGGATTCCGGTTGAACCAACATATGGCGGTATTTCACCAg                                              <  1:2726192/53‑1 (MQ=39)
                                     tttttATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGaa                                            >  1:926047/1‑54 (MQ=255)
                                        ttATCTGTCAGGATTCCGGTGGAACCAACATATGGCGCTATTTCACCAGAATGTCATTCTGCCGTTCTGCt                        >  1:569551/1‑71 (MQ=255)
                                           tCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCatt                      <  1:1999415/70‑1 (MQ=255)
                                                 aGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTg                >  1:3568613/1‑70 (MQ=255)
                                                 aGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTg                >  1:4468016/1‑70 (MQ=255)
                                                 aGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTg                >  1:1160957/1‑70 (MQ=255)
                                                  ggATTCCGGTGGAACCAACATATTGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa              <  1:2874916/71‑1 (MQ=255)
                                                  ggATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa              <  1:2461501/71‑1 (MQ=255)
                                                    aTTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCaaa            >  1:4535104/1‑71 (MQ=255)
                                                     ttCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGt                                         <  1:4217345/41‑1 (MQ=255)
                                                     ttCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGt                                         <  1:4313196/41‑1 (MQ=255)
                                                       ccGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCAt                                      >  1:343071/1‑42 (MQ=255)
                                                          gTGGAACCAACATATGGCGGTATTTCACCAGAATGTCa                                       <  1:1560350/38‑1 (MQ=255)
                                                           tGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa              <  1:2224550/62‑1 (MQ=255)
                                                            ggAACCAACATATGGCGGTATTTCACCAGAATGTCAt                                      <  1:3489907/37‑1 (MQ=255)
                                                              aaCCAACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc  <  1:547276/71‑1 (MQ=255)
                                                                  |                                                                  
CGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAC  >  W3110S.gb/1101045‑1101177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: