BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,189,339	A→G	L160S (TTA→TCA)	ycfD ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,189,339	0	A	G	100.0%	109.5 / NA	33	L160S (TTA→TCA)	ycfD	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/16); total (17/16)

GAATATAAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGCGA  >  W3110S.gb/1189274‑1189398

gAATATAAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGa                                                        <  1:1091431/71‑1 (MQ=255)

   tataAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATcc                                                     >  1:80046/1‑71 (MQ=255)

     taAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCg                                                    <  1:959739/70‑1 (MQ=255)

     taAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCg                                                    >  1:3936020/1‑70 (MQ=255)

     taAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCg                                                    >  1:3698406/1‑70 (MQ=255)

     taAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCg                                                    >  1:2919168/1‑70 (MQ=255)

     taAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCg                                                    >  1:2410127/1‑70 (MQ=255)

       aaGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa                                                  <  1:14055/70‑1 (MQ=255)

       aaGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa                                                  <  1:338537/70‑1 (MQ=255)

            tatCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTggg                                            >  1:2718101/1‑71 (MQ=255)

             atCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTggg                                            >  1:3468989/1‑70 (MQ=255)

              tCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTggg                                            >  1:307440/1‑69 (MQ=255)

              tCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGc                                           <  1:2085733/70‑1 (MQ=255)

                aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGt                                        >  1:388163/1‑71 (MQ=255)

                       tCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGttt                                 >  1:373678/1‑71 (MQ=255)

                       tCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGttt                                 >  1:3946874/1‑71 (MQ=255)

                       tCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGtt                                  >  1:587751/1‑70 (MQ=255)

                         cAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGttt                                 <  1:3287727/69‑1 (MQ=255)

                         cAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCa                               <  1:1643589/71‑1 (MQ=255)

                         cAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCa                               <  1:4581786/71‑1 (MQ=255)

                            ctctTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGa                                                        <  1:1086890/43‑1 (MQ=255)

                            ctctTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGa                                                        <  1:2836484/43‑1 (MQ=255)

                              ctTCATCGATGATGGCTTCGAACGGATCTACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTg                           >  1:367225/1‑70 (MQ=255)

                                   tCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgtg                                              <  1:36692/46‑1 (MQ=255)

                                   tCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgtg                                              <  1:1298868/46‑1 (MQ=255)

                                   tCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCtt                     <  1:3287227/71‑1 (MQ=255)

                                    cGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa                                                  <  1:1168160/41‑1 (MQ=255)

                                            gCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCa                                          >  1:4584721/1‑41 (MQ=255)

                                               tCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCg         >  1:3826837/1‑71 (MQ=255)

                                                   aCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAg     >  1:1999195/1‑71 (MQ=255)

                                                    cGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGc    >  1:3729316/1‑71 (MQ=255)

                                                     ggATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGc    <  1:1502911/70‑1 (MQ=255)

                                                      gATCGACCTGTGACAGATCCGGATGTGTGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGcga  <  1:4123974/71‑1 (MQ=255)

GAATATAAAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGCGA  >  W3110S.gb/1189274‑1189398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`