Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,207,066	A→G	E440E (GAA→GAG)	ymfN →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,207,066	0	A	G	100.0%	132.1 / NA	40	E440E (GAA→GAG)	ymfN	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/18);  total (22/18)

GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAT  >  W3110S.gb/1206998‑1207130                                                                     |                                                                 gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga                                                                <  1:3267804/71‑1 (MQ=255) gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga                                                                <  1:2824496/71‑1 (MQ=255)    aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGacac                                                             >  1:2227914/1‑71 (MQ=255)     aCAAAATCTATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACg                                                            <  1:1584537/71‑1 (MQ=255)             gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTg                                                     <  1:3345392/70‑1 (MQ=255)             gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                                    >  1:2628342/1‑71 (MQ=255)             gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                                    >  1:356348/1‑71 (MQ=255)             gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                                    >  1:232885/1‑71 (MQ=255)             gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                                    >  1:4325069/1‑71 (MQ=255)                 gTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATca                                                 <  1:843922/70‑1 (MQ=255)                 gTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCT‑ATcac                                                <  1:2261683/70‑1 (MQ=255)                  tGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTg                                                         <  1:3750965/61‑1 (MQ=255)                       ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcaca                                               <  1:3111144/66‑1 (MQ=255)                       ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAg                                          <  1:4429475/71‑1 (MQ=255)                        tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGTACACGCTGTCTGa                                                    >  1:4317417/1‑60 (MQ=255)                        tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa                                                    >  1:4330625/1‑60 (MQ=255)                        tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGt                                         >  1:54204/1‑71 (MQ=255)                        tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGt                                         >  1:3087736/1‑71 (MQ=255)                         gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga                                                                >  1:2067380/1‑47 (MQ=255)                         gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTc                                        >  1:1885054/1‑71 (MQ=255)                         gagcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGt                                         <  1:2394977/68‑1 (MQ=255)                          cgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTcc                                       <  1:755445/71‑1 (MQ=255)                                  aTGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGAt                               >  1:424008/1‑71 (MQ=255)                                  aTGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGAt                               >  1:1787661/1‑71 (MQ=255)                                         ttGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcg                        <  1:3252348/71‑1 (MQ=255)                                           ggCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgct                      >  1:3814752/1‑71 (MQ=255)                                            gCAGAGCCATGCTTTACGAGAAAGAGGACACTCTGTCTGATCACATTGAg                                          <  1:3195040/50‑1 (MQ=38)                                             cAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt                    <  1:4281442/71‑1 (MQ=255)                                             cAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt                    <  1:4234019/71‑1 (MQ=255)                                              agatCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTa                   <  1:1444319/71‑1 (MQ=255)                                                 gCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACt                >  1:486276/1‑71 (MQ=255)                                                  ccATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACt                >  1:3921256/1‑70 (MQ=255)                                                     tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttaa                   <  1:2897243/64‑3 (MQ=255)                                                     tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTa                   <  1:3194369/64‑1 (MQ=255)                                                     tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTg               <  1:1821360/68‑1 (MQ=255)                                                       cTGTACGAGAAAGAGGACACGCTGTCTGCTCACATTGAGTCCTACGGGATCcgc                           >  1:595208/1‑54 (MQ=255)                                                       cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgc                           >  1:712811/1‑54 (MQ=255)                                                       cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa                  >  1:4570254/1‑63 (MQ=255)                                                       cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa                  >  1:4571359/1‑63 (MQ=255)                                                       cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGt           >  1:2063957/1‑70 (MQ=255)                                                          tACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAt        >  1:416479/1‑70 (MQ=255)                                                            cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTAt     >  1:291220/1‑71 (MQ=255)                                                            cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTAt     <  1:1977250/71‑1 (MQ=255)                                                             gacaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATg    <  1:4286260/68‑1 (MQ=255)                                                               gaaagagGACACGCTTTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  <  1:2898969/71‑1 (MQ=255)                                                               gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  >  1:3434028/1‑71 (MQ=255)                                                               gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  <  1:1136728/71‑1 (MQ=255)                                                               gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  <  1:2084011/71‑1 (MQ=255)                                                               gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  <  1:326138/71‑1 (MQ=255)                                                                 aagaggACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt  <  1:1720908/69‑1 (MQ=255)                                                                     |                                                                 GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAT  >  W3110S.gb/1206998‑1207130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑