Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,352,814	G→A	intergenic (‑61/+307)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,352,814	0	G	A	100.0%	139.1 / NA	47	intergenic (‑61/+307)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

AAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGT  >  W3110S.gb/1352746‑1352881                                                                     |                                                                    aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATa                                                                   <  1:3353164/71‑1 (MQ=255) aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATa                                                                   <  1:4259518/71‑1 (MQ=255)      cATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAg                                                              <  1:613040/71‑1 (MQ=255)        tAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATaaa                                                                 <  1:2371910/66‑1 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:1019874/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:3178011/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:2505079/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:2431216/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:2372192/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:2351161/1‑70 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACg                                                          <  1:2272123/71‑1 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACg                                                          <  1:2982801/71‑1 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACg                                                          <  1:3117910/71‑1 (MQ=255)          gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTAGTTTTAATAAACAGTAc                                                           >  1:792427/1‑70 (MQ=255)          gCTTTAATCCTTATTCTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTAc                                                           >  1:4440374/1‑70 (MQ=255)             ttAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTa                                                            >  1:1777030/1‑66 (MQ=255)                 tCCTTATT‑TTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATa                                                  <  1:2132807/71‑1 (MQ=255)                   cTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACa                                                      <  1:1543690/66‑1 (MQ=255)                   cTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACa                                                      <  1:4282714/66‑1 (MQ=255)                   cTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATa                                                  <  1:3611406/70‑1 (MQ=255)                       ttgttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACa                                                      <  1:4036206/62‑1 (MQ=255)                         gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGt                                            <  1:1646174/70‑1 (MQ=255)                         gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGt                                            <  1:1480109/70‑1 (MQ=255)                         gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGt                                            >  1:697240/1‑70 (MQ=255)                            gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGttat                                         >  1:3716049/1‑70 (MQ=255)                            gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGttat                                         >  1:948669/1‑70 (MQ=255)                              tGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGttatta                                       >  1:1369509/1‑70 (MQ=255)                              tGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGttatta                                       >  1:1903009/1‑70 (MQ=255)                                    ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCa                                 <  1:4049868/70‑1 (MQ=255)                                    ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCa                                 <  1:2692190/70‑1 (MQ=255)                                    ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCa                                 <  1:4281424/70‑1 (MQ=255)                                    ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCa                                 <  1:3036360/70‑1 (MQ=255)                                    ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCa                                 <  1:1195165/70‑1 (MQ=255)                                      gtgCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAg                                                              >  1:1503971/1‑39 (MQ=255)                                      gtgCCTGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAAcc                              >  1:4482129/1‑71 (MQ=255)                                           tGAAAATCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAAc                               >  1:2994466/1‑65 (MQ=255)                                             aaaaTCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACctgtctgt                       <  1:14852/71‑1 (MQ=255)                                               aaTCAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCtgt                       >  1:3620916/1‑69 (MQ=255)                                                  cAGGCGATTCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAg                    >  1:3868007/1‑69 (MQ=255)                                                         ttCGTCGTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATc                                  <  1:747948/48‑1 (MQ=255)                                                          tcgtcgTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAg                    >  1:988308/1‑61 (MQ=255)                                                          tcgtcgTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGt          >  1:1249022/1‑71 (MQ=255)                                                          tcgtcgTTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGt          >  1:1177342/1‑71 (MQ=255)                                                           cgtcgtTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGtt         >  1:1381967/1‑71 (MQ=255)                                                            gtcgtTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGtt         <  1:4142545/70‑1 (MQ=255)                                                            gtcgtTTTAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGtt         <  1:2834095/70‑1 (MQ=255)                                                                  ttAATAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGt  >  1:325953/1‑71 (MQ=255)                                                                     |                                                                    AAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGT  >  W3110S.gb/1352746‑1352881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑