Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,400,571	G→A	L224L (CTG→TTG)	fnr ←	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,400,571	0	G	A	100.0%	92.7 / NA	33	L224L (CTG→TTG)	fnr	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/11);  total (22/11)

TGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA  >  W3110S.gb/1400507‑1400638                                                                 |                                                                    tGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTA‑TTACCTTTGACTGCCAACa                                                                   >  1:606048/1‑66 (MQ=255)   aCCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGc                                                             >  1:4507357/1‑71 (MQ=255)   aCCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGc                                                             >  1:811544/1‑71 (MQ=255)                      gcATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcag                                          >  1:2589799/1‑71 (MQ=255)                      gcATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACAcag                                          >  1:3310013/1‑71 (MQ=255)                        aTCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca                                        <  1:56424/71‑1 (MQ=255)                         tCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcag                                          >  1:3408122/1‑68 (MQ=255)                           gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGAc                                     >  1:1069251/1‑71 (MQ=255)                           gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGAc                                     >  1:1965018/1‑71 (MQ=255)                           gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGAc                                     >  1:2103485/1‑71 (MQ=255)                            ttATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACg                                    <  1:2774747/71‑1 (MQ=255)                            ttATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACg                                    <  1:2044916/71‑1 (MQ=255)                                 ttCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGa                               >  1:4525295/1‑71 (MQ=255)                                        gTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTc                        >  1:2972746/1‑71 (MQ=255)                                            tGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca                                        <  1:4234541/51‑1 (MQ=255)                                             gTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCg                   >  1:2481120/1‑71 (MQ=255)                                              tATTTACCTTTGCCTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt                  >  1:4058309/1‑71 (MQ=255)                                              tATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGa                                      >  1:628288/1‑51 (MQ=255)                                              tATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt                  >  1:632532/1‑71 (MQ=255)                                                tttACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATg                             <  1:779275/58‑1 (MQ=255)                                                tttACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt                  >  1:4033659/1‑69 (MQ=255)                                                  tACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAgg              >  1:2425274/1‑71 (MQ=255)                                                  tACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAg               >  1:1567044/1‑70 (MQ=255)                                                       ttGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc            >  1:2529114/1‑68 (MQ=255)                                                       ttGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc            >  1:161836/1‑68 (MQ=255)                                                        tGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCa          <  1:3687530/69‑1 (MQ=255)                                                        tGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCa          <  1:3255994/69‑1 (MQ=255)                                                          aCTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGAt       <  1:1995576/70‑1 (MQ=255)                                                          aCTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCACAGAt       <  1:2416664/70‑1 (MQ=255)                                                          aCTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGAAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATa      <  1:873378/71‑1 (MQ=255)                                                              ccAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTa  <  1:2767871/71‑1 (MQ=255)                                                              ccAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTa  >  1:216073/1‑71 (MQ=255)                                                              ccAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTa  >  1:1319978/1‑71 (MQ=255)                                                                 |                                                                    TGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA  >  W3110S.gb/1400507‑1400638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑