Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	172,490	A→G	E343E (GAA→GAG)	fhuB →	fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	172,490	0	A	G	100.0%	252.9 / NA	73	E343E (GAA→GAG)	fhuB	fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/38);  total (35/38)

GCTGTTGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTGTGGTGGTGGC  >  W3110S.gb/172424‑172555                                                                   |                                                                  gCTGTTGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAgcgc                                                                >  1:3851527/1‑70 (MQ=255) gCTGTTGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAgcgc                                                                >  1:4658228/1‑70 (MQ=255) gCTGTTGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAgcgc                                                                >  1:544823/1‑70 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:1035445/69‑1 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:3510214/69‑1 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:3031941/69‑1 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:2180192/69‑1 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:1726315/69‑1 (MQ=255)     ttGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCaa                                                             <  1:1003981/69‑1 (MQ=255)               tACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACa                                                           >  1:2712640/1‑61 (MQ=255)               tACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 >  1:782347/1‑71 (MQ=255)               tACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 >  1:2567827/1‑71 (MQ=255)               tACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 >  1:1850554/1‑71 (MQ=255)               tACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 >  1:718770/1‑71 (MQ=255)                aCGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 <  1:569879/70‑1 (MQ=255)                aCGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCg                                                 <  1:65192/70‑1 (MQ=255)                   cagcaTTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTg                                             <  1:18268/71‑1 (MQ=255)                   cagcaTTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTg                                             <  1:1531864/71‑1 (MQ=255)                        ttAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTc                                        >  1:4616966/1‑71 (MQ=255)                          agagcgcCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGACAACATGTGCTGGCGttt                                              >  1:4682133/4‑63 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:3337184/1‑54 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGcc                                           >  1:2092051/1‑66 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:4624260/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:452838/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:3120323/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:1746308/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:2515114/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:3579118/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcgc                                      >  1:3246760/1‑71 (MQ=255)                          aGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTcg                                       >  1:3311929/1‑70 (MQ=255)                                     tatGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGtgc                           <  1:764717/71‑1 (MQ=255)                                     tatGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGtgc                           >  1:4615182/1‑71 (MQ=255)                                       tGAAGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGtgctg                         >  1:2731433/1‑71 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:1363224/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:2870203/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:4073714/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:4293229/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:4298352/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACAtgtg                                                       >  1:2159096/1‑39 (MQ=255)                                         aaGGTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTgctgct                       <  1:3190025/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:4017055/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:4022463/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:3783223/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:945232/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:3396665/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1093751/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1414266/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1546379/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1612353/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1835910/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:2589519/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:3459864/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCCGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:1495590/71‑1 (MQ=255)                                            gTCAACGATCGTGTCGCCGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:513155/71‑1 (MQ=255)                                             tCAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGtt                    <  1:4685366/70‑1 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGc                                                      >  1:2851018/1‑35 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGc                                                      >  1:3060227/1‑35 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGtgct                          >  1:1062178/1‑63 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGa                  >  1:1545117/1‑71 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGa                  >  1:2469589/1‑71 (MQ=255)                                              cAACGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGa                  >  1:2520645/1‑71 (MQ=255)                                               aaCGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGGTGCTGTTGa                  <  1:588932/70‑1 (MQ=255)                                               aaCGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGa                  <  1:2764576/70‑1 (MQ=255)                                               aaCGATCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGa                  <  1:4211581/70‑1 (MQ=255)                                                   aTCGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTgctgct                       <  1:4625402/61‑1 (MQ=255)                                                   aTCGTGTCGCGGCTGAGCGCAAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTgctgct                       <  1:3799609/61‑1 (MQ=255)                                                     cGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGt                             >  1:1157740/1‑53 (MQ=255)                                                     cGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTg                   >  1:3215032/1‑63 (MQ=255)                                                     cGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCtgt           >  1:1965208/1‑71 (MQ=255)                                                     cGTGTCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCtgt           >  1:1226814/1‑71 (MQ=255)                                                       tgtCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtgg         <  1:590519/71‑1 (MQ=255)                                                         tCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggt        <  1:1861657/70‑1 (MQ=255)                                                         tCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggt        <  1:329238/70‑1 (MQ=255)                                                         tCGCGGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTAGCGGGCGGCGTGCTGCTGTTGATGGCTgtggt        <  1:1222590/70‑1 (MQ=255)                                                           gcgGCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTgtggtggt     <  1:389819/71‑1 (MQ=255)                                                              gCTGAGCGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTGTGGTGGTGgc  <  1:3521332/71‑1 (MQ=255)                                                                   |                                                                  GCTGTTGCCGCGTTTACGCAGCATTAGCGCGCCGGATATGAAGGTCAACGATCGTGTCGCGGCTGAACGCCAACATGTGCTGGCGTTTGCCCTCGCGGGCGGCGTGCTGCTGTTGATGGCTGTGGTGGTGGC  >  W3110S.gb/172424‑172555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑