Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,482,053	A→G	D401G (GAT→GGT)	ynbC →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,482,053	0	A	G	100.0%	143.3 / NA	42	D401G (GAT→GGT)	ynbC	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/23);  total (19/23)

TGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTG  >  W3110S.gb/1481985‑1482120                                                                     |                                                                    tgtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtc                                                                   >  1:3882302/1‑71 (MQ=255) tgtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtc                                                                   >  1:1761978/1‑71 (MQ=255)   ttGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc                                                                 <  1:305712/71‑1 (MQ=255)   ttGGCTGGCGCGGTATTCGCCAGCCCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc                                                                 >  1:719693/1‑71 (MQ=255)    tggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc                                                                 <  1:1874006/70‑1 (MQ=255)     ggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTcc                                                                <  1:1103031/70‑1 (MQ=255)     ggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCa                                                               >  1:1968179/1‑71 (MQ=255)              ggTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg                                                      <  1:3586554/71‑1 (MQ=255)              ggTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg                                                      <  1:1723460/71‑1 (MQ=255)               gTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg                                                      <  1:1766271/70‑1 (MQ=255)               gTATTCGCCAGCGAAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGt                                                     <  1:298851/71‑1 (MQ=255)                  ttCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGttt                                                   <  1:33109/70‑1 (MQ=255)                    cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc                                                >  1:1700244/1‑71 (MQ=255)                    cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc                                                >  1:818457/1‑71 (MQ=255)                    cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc                                                >  1:2332008/1‑71 (MQ=255)                    cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc                                                >  1:1371093/1‑71 (MQ=255)                           gcAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCcgc                                         >  1:1258604/1‑71 (MQ=255)                              aaaCCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTg                                      <  1:103372/71‑1 (MQ=255)                                   cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                 <  1:2683385/71‑1 (MQ=255)                                   cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                 <  1:2805666/71‑1 (MQ=255)                                   cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                 <  1:329035/71‑1 (MQ=255)                                   cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                 <  1:1573167/71‑1 (MQ=255)                                    aTTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGAc                                <  1:161675/71‑1 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACa                               >  1:206554/1‑69 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACa                               >  1:2993540/1‑69 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                             >  1:958889/1‑71 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                             >  1:426055/1‑71 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                             >  1:3463456/1‑71 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                             >  1:413168/1‑71 (MQ=255)                                       tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                             >  1:3825863/1‑71 (MQ=255)                                        aCAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTg                            >  1:2457379/1‑71 (MQ=255)                                           aaTACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                 <  1:2932974/63‑1 (MQ=255)                                               cTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAgg                     >  1:152509/1‑71 (MQ=255)                                                  aTTAAACAAGCCGTTGCCGGTCTCCATGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAggg                    <  1:1586801/69‑1 (MQ=255)                                                  aTTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGc                   <  1:2868740/70‑1 (MQ=255)                                                    tAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATg                >  1:4673894/1‑71 (MQ=255)                                                         aaGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGgcgc           <  1:3271242/71‑1 (MQ=255)                                                           gCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTa         <  1:3751755/71‑1 (MQ=255)                                                             cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGTGCGCTATg       <  1:535855/71‑1 (MQ=255)                                                             cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATg       <  1:3499953/71‑1 (MQ=255)                                                             cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATg       <  1:4214294/71‑1 (MQ=255)                                                                  ccGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTg  <  1:862853/71‑1 (MQ=255)                                                                     |                                                                    TGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTG  >  W3110S.gb/1481985‑1482120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑