Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,533,715	T→C	G62G (GGT→GGC)	ydcC →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,533,715	0	T	C	100.0%	89.3 / NA	29	G62G (GGT→GGC)	ydcC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

TTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA  >  W3110S.gb/1533678‑1533781                                      |                                                                   ttGGGGAAACACATCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGacac                                    <  1:1294514/70‑1 (MQ=25) ttGGGGAAACACATCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGacac                                    <  1:3926767/70‑1 (MQ=25)  tGGGGAAACACATCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGacac                                    <  1:3397263/69‑1 (MQ=25)   ggggAAACACATCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCa                                  <  1:814015/70‑1 (MQ=25)         acacATCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGc                              >  1:4046857/1‑68 (MQ=255)           acaTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCagag                         >  1:1502559/1‑71 (MQ=255)           acaTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCagag                         >  1:2126958/1‑71 (MQ=255)           acaTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCa                            >  1:3289086/1‑68 (MQ=255)            caTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGt                        >  1:177468/1‑71 (MQ=255)              tCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTg                      <  1:1561451/71‑1 (MQ=255)              tCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTg                      <  1:1342093/71‑1 (MQ=255)                ccGATTTTTTGAAGCAATATTGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTa                    <  1:3765508/71‑1 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGt                     <  1:4082374/70‑1 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGt                     <  1:3406772/70‑1 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTa                    >  1:4373222/1‑71 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTa                    >  1:55484/1‑71 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTa                    >  1:686765/1‑71 (MQ=255)                ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGACAGAGTTGTa                    >  1:4365245/1‑71 (MQ=255)                 cGATTTTTTGAAGCAATATGGCGTTTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGt                     <  1:2709348/69‑1 (MQ=255)                 cGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTAt                   >  1:1362899/1‑71 (MQ=255)                   aTTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATcc                 <  1:3180518/71‑1 (MQ=255)                               aaTATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCt     >  1:1522765/1‑71 (MQ=21)                               aaTATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCt     >  1:1915371/1‑71 (MQ=21)                                  atGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGc   >  1:447415/1‑70 (MQ=25)                                   tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCa                                  >  1:4225429/1‑38 (MQ=255)                                   tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGc                              <  1:2855279/42‑1 (MQ=255)                                   tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTa                    >  1:3820478/1‑52 (MQ=255)                                   tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa  >  1:1960444/1‑70 (MQ=25)                                   tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa  >  1:1148254/1‑70 (MQ=25)                                      |                                                                   TTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA  >  W3110S.gb/1533678‑1533781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑