Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,558,725	G→A	Q129Q (CAG→CAA)	osmC →	osmotically inducible, stress‑inducible membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,558,725	0	G	A	100.0%	157.6 / NA	53	Q129Q (CAG→CAA)	osmC	osmotically inducible, stress‑inducible membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/25);  total (28/25)

CCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGT  >  W3110S.gb/1558657‑1558790                                                                     |                                                                  ccGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGt                                                                 <  1:751232/71‑1 (MQ=255)      tATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGGAAAAGCAGGATGCCCGGTCTCTCAAGTACTg                                                             >  1:1006134/1‑70 (MQ=255)          gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc                                                        >  1:3227187/1‑71 (MQ=255)          gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc                                                        >  1:2443679/1‑71 (MQ=255)          gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc                                                        >  1:2041848/1‑71 (MQ=255)              ccTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGa                                                     <  1:3062058/70‑1 (MQ=255)              ccTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGa                                                     <  1:3745855/70‑1 (MQ=255)                       ttGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGaaa                                                   >  1:4449837/1‑63 (MQ=255)                         gACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTgg                                          <  1:936911/70‑1 (MQ=255)                         gACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTgg                                          <  1:1717538/70‑1 (MQ=255)                             gCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAG‑GGAAATTACGCTGGATTAc                                     >  1:3392075/1‑70 (MQ=255)                              cATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTAc                                     >  1:2297098/1‑70 (MQ=255)                               aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGaaa                                                   >  1:347885/1‑55 (MQ=255)                               aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTAATGAAAGCGGaaa                                                   >  1:3119644/1‑55 (MQ=255)                               aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGAACTGAAAGCGGAAATTACGCTGGATTAc                                     >  1:995106/1‑69 (MQ=255)                                tAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTAcc                                    <  1:4295262/69‑1 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCa                                   >  1:4684806/1‑68 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:2409474/1‑71 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:3043520/1‑71 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:1201143/1‑71 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:1267026/1‑71 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:899856/1‑71 (MQ=255)                                  aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt                                >  1:1838079/1‑71 (MQ=255)                                     cAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGa                              >  1:2398882/1‑70 (MQ=255)                                        aaagcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAAt                           <  1:3736253/70‑1 (MQ=255)                                          agcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCgg                                                      >  1:1631229/1‑41 (MQ=255)                                           gcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTa                       <  1:1094997/71‑1 (MQ=255)                                             aaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTaaa                     <  1:2499269/71‑1 (MQ=255)                                              aaagcaGGATGCCCGGTCTCTCAAGTACTGTAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:2201309/71‑1 (MQ=255)                                              aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:3159243/71‑1 (MQ=255)                                              aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:267738/71‑1 (MQ=255)                                              aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:1491440/71‑1 (MQ=255)                                              aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:2078168/71‑1 (MQ=255)                                               aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:313351/71‑1 (MQ=255)                                               aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:1274658/71‑1 (MQ=255)                                               aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:1777329/71‑1 (MQ=255)                                               aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:1854448/71‑1 (MQ=255)                                               aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:3739965/71‑1 (MQ=255)                                                agcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   >  1:106644/1‑70 (MQ=255)                                                     gATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:3629840/65‑1 (MQ=255)                                                     gATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:4031085/65‑1 (MQ=255)                                                      aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                    <  1:3698931/63‑1 (MQ=255)                                                      aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCg            <  1:3524455/71‑1 (MQ=255)                                                      aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCg            >  1:1925349/1‑71 (MQ=255)                                                         cccGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGAt         <  1:2125910/71‑1 (MQ=255)                                                             gTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc                   <  1:3292651/57‑1 (MQ=255)                                                               ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg   >  1:2288871/1‑71 (MQ=255)                                                               ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg   >  1:1846102/1‑71 (MQ=255)                                                               ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg   >  1:2302638/1‑71 (MQ=255)                                                               ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg   >  1:2614091/1‑71 (MQ=255)                                                               ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg   >  1:3107951/1‑71 (MQ=255)                                                                tctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCa                  >  1:4343352/1‑55 (MQ=255)                                                                tctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGt  >  1:4121149/1‑71 (MQ=255)                                                                     |                                                                  CCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGT  >  W3110S.gb/1558657‑1558790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑