| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,646,707 | C→T | intergenic (‑91/+126) | rem ← / ← hokD | hypothetical protein/small toxic polypeptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,646,707 | 0 | C | T | 100.0% | 52.7 / NA | 19 | intergenic (‑91/+126) | rem/hokD | hypothetical protein/small toxic polypeptide |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/7); total (12/7) | |||||||||||
TTTAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGG > W3110S.gb/1646647‑1646772 | tttAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTAc < 1:712285/63‑1 (MQ=255) tAAGGGATCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTACCGTCCAGAAc > 1:2396174/1‑71 (MQ=255) cccTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTAc > 1:2506586/1‑50 (MQ=255) cccTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTAc > 1:4415551/1‑50 (MQ=255) ccTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTACCGTCCAGAACTTCAAAc > 1:3169132/1‑66 (MQ=255) cTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGTACCGTCCAGAACTTCAAACACGTTGAAAATaaaa < 1:951090/71‑1 (MQ=255) cgcgcTACTTAAGTATTTTTGATTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATaaaaac > 1:2191390/1‑70 (MQ=255) cgcTACTTAAGTATTTTTGATTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATaaaaacaaa < 1:1683645/71‑1 (MQ=255) tttttGATTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATaaaaacaaa > 1:447285/1‑58 (MQ=255) tttttGATTCTATTCCGGTACCGTCCAGAAATTCa > 1:1314384/1‑35 (MQ=25) tttGATTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCgg > 1:788287/1‑71 (MQ=255) aTTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTa < 1:97913/71‑1 (MQ=255) aTTCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTa < 1:3815235/71‑1 (MQ=255) ttCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTa < 1:2651495/70‑1 (MQ=255) tCTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATaaaaacaaa > 1:186913/1‑50 (MQ=255) cTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGt > 1:4212718/1‑71 (MQ=255) cTATTCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGt > 1:462263/1‑71 (MQ=255) tCCGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGc < 1:3849311/69‑1 (MQ=255) ccGGTACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCgg > 1:721796/1‑70 (MQ=255) | TTTAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGG > W3110S.gb/1646647‑1646772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |