| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,811,850 | G→A | intergenic (+88/‑75) | yniC → / → ydjM | predicted hydrolase/predicted inner membrane protein regulated by LexA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,811,850 | 0 | G | A | 97.4% | 111.1 / ‑3.8 | 39 | intergenic (+88/‑75) | yniC/ydjM | predicted hydrolase/predicted inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/16); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTA > W3110S.gb/1811784‑1811912 | cTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac < 1:2659988/71‑1 (MQ=255) ccTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGt > 1:2784983/1‑71 (MQ=255) ccTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGt < 1:224465/71‑1 (MQ=255) ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:794314/1‑71 (MQ=255) ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTca > 1:4518059/1‑70 (MQ=255) ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTca > 1:2840254/1‑70 (MQ=255) ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTc > 1:3780165/1‑69 (MQ=255) gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac < 1:3310134/70‑1 (MQ=255) gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:4586793/1‑70 (MQ=255) gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:3432059/1‑70 (MQ=255) tCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTa > 1:4048332/1‑59 (MQ=255) tCTTTATCTGAAATTGCATT‑AACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTa > 1:4070978/1‑58 (MQ=255) cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACt > 1:2706661/1‑71 (MQ=255) tATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTcc > 1:4110264/1‑71 (MQ=255) tGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGTAAGAATTACACCGTCACCACTTCCGTg > 1:755018/1‑70 (MQ=255) aaaTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa < 1:2391587/70‑1 (MQ=255) aaaTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTg < 1:2020062/68‑1 (MQ=255) aTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTg > 1:1184802/1‑71 (MQ=255) aTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTg > 1:3207239/1‑71 (MQ=255) gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:3152338/1‑70 (MQ=255) gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:1956926/1‑70 (MQ=255) gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:1910147/1‑70 (MQ=255) cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTaca > 1:1282634/1‑41 (MQ=255) cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTaca > 1:4677600/1‑41 (MQ=255) cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATaaaaa > 1:80453/1‑71 (MQ=255) cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATaaaaa > 1:4347940/1‑71 (MQ=255) cTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc < 1:3710219/71‑1 (MQ=255) cTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc < 1:4592814/71‑1 (MQ=255) aCGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:3991247/70‑1 (MQ=255) cGGATTAATCGTCAATTTAAGAGTAAGAATTACTCCGTCTCCACTTCCGTGc < 1:1968171/52‑1 (MQ=255) ggATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc < 1:2775090/71‑1 (MQ=255) aTTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTg < 1:1166711/71‑1 (MQ=255) tCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:3495182/61‑1 (MQ=255) gTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTAt > 1:2842856/1‑71 (MQ=255) agagAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:2942187/71‑1 (MQ=255) agagAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:2863066/71‑1 (MQ=255) agagAAAGAATTACACCGTCACCACTTCCGTCCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:641564/71‑1 (MQ=255) gagaAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa < 1:3884860/71‑1 (MQ=255) aaaGAATTACACCGTCACCACTTCCGTGCACTGTATaaa < 1:2075538/39‑1 (MQ=255) agaaTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa > 1:2277466/1‑51 (MQ=255) | CTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTA > W3110S.gb/1811784‑1811912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |