BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,832,108	C→T	T124T (ACG→ACA)	astA ←	arginine succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,832,108	0	C	T	100.0%	145.6 / NA	50	T124T (ACG→ACA)	astA	arginine succinyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/31); total (19/31)

AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGA  >  W3110S.gb/1832040‑1832172

aacataaaGCGCGATTTCGAGAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:1652578/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCTGGTCGAGAAACAgtgt                                                                >  1:2612846/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:3740203/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:2745998/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:4147610/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:4390773/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:1855112/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:4094256/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:4575798/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:1734202/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:1498006/1‑71 (MQ=255)

aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt                                                                >  1:148506/1‑71 (MQ=255)

 acataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGtgtg                                                               <  1:645339/71‑1 (MQ=255)

    taaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGcac                                                            <  1:3093760/71‑1 (MQ=255)

     aaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGca                                                             >  1:3848557/1‑69 (MQ=255)

     aaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGca                                                             >  1:2761609/1‑69 (MQ=255)

            gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct                                                    <  1:3966482/71‑1 (MQ=255)

            gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct                                                    <  1:3133522/71‑1 (MQ=255)

            gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct                                                    <  1:3571343/71‑1 (MQ=255)

               ttagACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGACACAGTGTGCACAGCTCgctgct                                                 >  1:15127/4‑71 (MQ=255)

               ttagACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgct                                                 >  1:3060412/4‑71 (MQ=255)

               ttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgc                                                  >  1:3345296/1‑70 (MQ=255)

                tCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGctgctg                                                >  1:4646575/1‑71 (MQ=255)

                 cGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgct                                                 <  1:1033205/69‑1 (MQ=255)

                        aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg                                        <  1:3019784/71‑1 (MQ=255)

                        aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg                                        <  1:2380358/71‑1 (MQ=255)

                        aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg                                        <  1:4422888/71‑1 (MQ=255)

                        aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg                                        <  1:3843079/71‑1 (MQ=255)

                           tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc                                     >  1:442951/1‑71 (MQ=255)

                           tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc                                     >  1:99190/1‑71 (MQ=255)

                           tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc                                     >  1:3348008/1‑71 (MQ=255)

                           tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc                                     >  1:2256208/1‑71 (MQ=255)

                            aGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCg                                    >  1:4339221/1‑71 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTTCCGGTGTGATCGt                                   <  1:127007/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:701034/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:4030902/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:1786277/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:4036657/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:3198789/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:4383260/71‑1 (MQ=255)

                             gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:4172882/71‑1 (MQ=255)

                              ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt                                  <  1:926233/71‑1 (MQ=255)

                              ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt                                  <  1:328785/71‑1 (MQ=255)

                              ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt                                  <  1:2266715/71‑1 (MQ=255)

                                   gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGAt                                      >  1:3499093/1‑62 (MQ=255)

                                     ccTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga                           <  1:635571/71‑1 (MQ=255)

                                      ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt                                  <  1:952727/63‑1 (MQ=255)

                                      ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga                           >  1:4214156/1‑70 (MQ=255)

                                      ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTCATCGtt                                  <  1:1810179/63‑1 (MQ=255)

                                       tctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTc                                                       <  1:2736423/41‑1 (MQ=255)

                                       tctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt                                   <  1:4613346/61‑1 (MQ=255)

                                         tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa                         >  1:4544769/1‑69 (MQ=255)

                                           tGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc                     >  1:3610692/1‑71 (MQ=255)

                                           tGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc                     >  1:4139328/1‑71 (MQ=255)

                                                cAGTCCGGGTAGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTAc                                >  1:4259934/1‑55 (MQ=255)

                                                 aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa                                 >  1:3478817/1‑53 (MQ=255)

                                                 aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc               <  1:1270420/71‑1 (MQ=255)

                                                 aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc               <  1:3805406/71‑1 (MQ=255)

                                                       ggTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCg         <  1:842572/71‑1 (MQ=255)

                                                        gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTTAGAAACAGCGTCGGCAATGCGt        <  1:4121295/71‑1 (MQ=255)

                                                        gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt        <  1:457593/71‑1 (MQ=255)

                                                         tCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt        >  1:4032972/1‑70 (MQ=255)

                                                              aaaCAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGa  <  1:1129093/71‑1 (MQ=255)

AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGA  >  W3110S.gb/1832040‑1832172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`