Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,835,870	C→T	A14V (GCT→GTT)	ydjY →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,835,870	0	C	T	100.0%	98.2 / NA	33	A14V (GCT→GTT)	ydjY	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/12);  total (21/12)

ACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAA  >  W3110S.gb/1835802‑1835935                                                                     |                                                                  aCTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTg                                                                 >  1:455654/1‑71 (MQ=255) aCTCACACAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTg                                                                 >  1:4370976/1‑71 (MQ=255)  cTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTg                                                                 <  1:449711/70‑1 (MQ=255)       ccAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCActct                                                           >  1:3684579/1‑71 (MQ=255)          aaaaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGtt                                                        <  1:2021192/71‑1 (MQ=255)          aaaaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGtt                                                        <  1:804649/71‑1 (MQ=255)          aaaaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGtt                                                        <  1:42713/71‑1 (MQ=255)          aaaaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGtt                                                        <  1:3587267/71‑1 (MQ=255)          aaaaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGtt                                                        <  1:3992103/71‑1 (MQ=255)            aaaTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTa                                                      <  1:971191/71‑1 (MQ=255)              aTGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTACt                                                    >  1:2428335/1‑71 (MQ=255)                                  gCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTg                                               >  1:4307995/1‑56 (MQ=255)                                  gCAACATTATTCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCg                                >  1:3922674/1‑71 (MQ=255)                                       attattCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGc                           >  1:1942467/1‑71 (MQ=255)                                          attCAGTGTCATGGAAAAAAGGACTGGTTGCGCTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                        >  1:844594/1‑71 (MQ=255)                                          attCAGTGTCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                        >  1:839121/1‑71 (MQ=255)                                                tgtCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTggg                                       <  1:2740467/50‑1 (MQ=255)                                                  tCATGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATcaacaa                  >  1:2306446/1‑69 (MQ=255)                                                    aTGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag               <  1:1475882/70‑1 (MQ=255)                                                    aTGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag               >  1:3828072/1‑70 (MQ=255)                                                    aTGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag               >  1:3619490/1‑70 (MQ=255)                                                    aTGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag               <  1:2782153/70‑1 (MQ=255)                                                    aTGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag               <  1:2211998/70‑1 (MQ=255)                                                     tGGAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATcaacaa                  <  1:1922561/66‑1 (MQ=255)                                                       gAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAaga                >  1:2623588/1‑66 (MQ=255)                                                       gAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATg           >  1:2780562/1‑71 (MQ=255)                                                       gAAAAAAGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATg           >  1:2775243/1‑71 (MQ=255)                                                          aaaaGGACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATgcgg        >  1:3605478/1‑71 (MQ=255)                                                               gACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                        >  1:111040/1‑50 (MQ=255)                                                               gACTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGaa             >  1:3076650/1‑61 (MQ=255)                                                                 cTGGTTGCACTCTGTTTACTGGCTGTTGCTTGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCa   >  1:821172/1‑69 (MQ=255)                                                                 cTGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCaa  >  1:2513684/1‑70 (MQ=255)                                                                  tGGTTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCaa  >  1:796610/1‑69 (MQ=255)                                                                     |                                                                  ACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAA  >  W3110S.gb/1835802‑1835935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑