Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,838,873	C→T	P363S (CCG→TCG)	ynjB →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,838,873	0	C	T	100.0%	101.3 / NA	36	P363S (CCG→TCG)	ynjB	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/28);  total (8/28)

GATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGC  >  W3110S.gb/1838810‑1838930                                                                |                                                          gATCCGCAAAAACTGCCTGACGGGCAGCTCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:2285006/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:4510790/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:173279/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:3827453/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:3219537/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:2345601/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:309735/70‑1 (MQ=255) gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt                                                    <  1:470523/71‑1 (MQ=255)         aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTAc                                                  >  1:4511503/1‑65 (MQ=255)         aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTAc                                                  >  1:2345803/1‑65 (MQ=255)         aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCt                                             >  1:2335584/1‑70 (MQ=255)          aaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATCCCGCAGGATCTGTCGCCGGTACTGGCTGa                                           <  1:3109716/71‑1 (MQ=255)               gCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt                                                    <  1:3041291/57‑1 (MQ=255)               gCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACcg                                       >  1:655931/1‑70 (MQ=255)                    aCGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg                                                     <  1:2159624/51‑1 (MQ=255)                      gggCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAgg                               >  1:1129084/1‑71 (MQ=255)                             gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa                        <  1:666350/71‑1 (MQ=255)                             gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa                        <  1:353980/71‑1 (MQ=255)                             gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa                        <  1:4206837/71‑1 (MQ=255)                              cgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt                                                    <  1:2174915/42‑1 (MQ=255)                                    aTTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAg                                <  1:3699199/56‑1 (MQ=255)                                     ttGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACcgcacgca                                 <  1:4342944/54‑1 (MQ=255)                                           tCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACa          <  1:4340540/71‑1 (MQ=255)                                            cAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAAc           >  1:3383232/1‑69 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:1406547/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:541307/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:52522/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:973055/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:3655769/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:3147932/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:2254587/71‑1 (MQ=255)                                             aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg        <  1:1587345/71‑1 (MQ=255)                                             aaGAATGCCGAAGGATCTGTCGCCGGTACTGGCTGAACCGCAAGCAGGTTGGGTAAATGCGCTGGAAc           >  1:604996/1‑68 (MQ=255)                                                  tGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAAt     <  1:2383628/69‑1 (MQ=255)                                                    ccGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGc  <  1:787443/70‑1 (MQ=255)                                                             cTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATgcgc                 >  1:3455581/1‑46 (MQ=255)                                                                |                                                          GATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGC  >  W3110S.gb/1838810‑1838930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑