Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,921,924 G→A intergenic (+67/‑13) yebT → / → yebU conserved hypothetical protein/predicted methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,921,9240GA100.0% 49.9 / NA 19intergenic (+67/‑13)yebT/yebUconserved hypothetical protein/predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/5);  total (14/5)

ATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGC  >  W3110S.gb/1921857‑1921985
                                                                   |                                                             
atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATgg                                                            >  1:1304488/1‑71 (MQ=255)
   cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTa                                                          <  1:736140/70‑1 (MQ=255)
         gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCGTGTTTTTTTGCCAATGGTACATGCTc                                                   >  1:4261233/1‑71 (MQ=255)
         gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTAc                                                         >  1:4095352/1‑65 (MQ=255)
                  tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTa                                                          <  1:377557/55‑1 (MQ=255)
                    ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAaca                                        >  1:374245/1‑71 (MQ=255)
                      tGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAacaca                                      >  1:712250/1‑71 (MQ=255)
                       gcgcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTgg                                               >  1:4527192/1‑61 (MQ=255)
                               gCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACa                                                        >  1:2784747/1‑44 (MQ=255)
                               gCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTAtt                             >  1:546678/1‑71 (MQ=255)
                                  tttATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTcc                          >  1:2154024/1‑71 (MQ=255)
                                    tATGCTAAACTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAacaca                                      >  1:2761798/1‑57 (MQ=255)
                                    tATGCTAAACTGCGCGCCTGTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg                        >  1:1533225/1‑70 (MQ=255)
                                           aaCTGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc                   <  1:2431408/69‑1 (MQ=255)
                                             ttGCGCGCCTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCGttt               >  1:1681983/2‑71 (MQ=255)
                                                     cTGTTTTTTTGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGAcaca       <  1:3442377/71‑1 (MQ=255)
                                                        tttttttGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc                   <  1:3592977/56‑1 (MQ=255)
                                                          tttttGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc              >  1:483493/1‑59 (MQ=255)
                                                          tttttGCCAATGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgc  >  1:748158/1‑71 (MQ=255)
                                                                   |                                                             
ATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGC  >  W3110S.gb/1921857‑1921985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: