Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,935,986	C→T	M111I (ATG→ATA)	edd ←	6‑phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,935,986	0	C	T	100.0%	184.7 / NA	61	M111I (ATG→ATA)	edd	6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/19);  total (42/19)

ATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT  >  W3110S.gb/1935918‑1936053                                                                     |                                                                    aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat                                                                   >  1:2954337/1‑71 (MQ=255) aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat                                                                   >  1:1514672/1‑71 (MQ=255) aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat                                                                   >  1:3981999/1‑71 (MQ=255) aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat                                                                   >  1:1869892/1‑71 (MQ=255) aTCGCTATAACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCATGCCCCTGGGTGACACCATCACatat                                                                   >  1:1337308/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:994839/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:4364383/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:4614747/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:4624928/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:550558/1‑71 (MQ=255)    gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc                                                                >  1:1781860/1‑71 (MQ=255)        tCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCgg                                                             <  1:321761/70‑1 (MQ=255)          aCTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGaa                                                           >  1:3253129/1‑70 (MQ=255)          aCTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGaa                                                           >  1:2600818/1‑70 (MQ=255)             tCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACAcc                                                       >  1:1406967/1‑71 (MQ=255)                   cTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACAccgcccgc                                                 <  1:4227057/71‑1 (MQ=255)                      agcagcGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCa                                                <  1:3069412/69‑1 (MQ=255)                      agcagcGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCa                                                <  1:3293108/69‑1 (MQ=255)                               aaTTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAAc                                     >  1:1557317/1‑71 (MQ=255)                               aaTTCCATTCCATCCTGCCCCTGGGTGACACCATAACATATCGCCGGAACACCGCCCGCAACCTGACCAAc                                     >  1:485061/1‑71 (MQ=255)                                 ttccattccaTCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg                                   <  1:3408597/71‑1 (MQ=255)                                  tccattccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg                                   >  1:3150925/1‑70 (MQ=255)                                  tccattccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg                                   >  1:1908057/1‑70 (MQ=255)                                      ttccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTg                                           <  1:650373/58‑1 (MQ=255)                                       tccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg                             >  1:2421472/1‑71 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGa                                          >  1:1802176/1‑57 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg                             <  1:1490276/70‑1 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg                             <  1:3164569/70‑1 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc                            >  1:1227348/1‑71 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc                            >  1:550387/1‑71 (MQ=255)                                        ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc                            >  1:903776/1‑71 (MQ=255)                                          atCCTGACCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCtt                          >  1:1720181/1‑71 (MQ=255)                                             cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt                       >  1:2290975/1‑71 (MQ=255)                                             cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt                       >  1:4517076/1‑71 (MQ=255)                                             cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt                       >  1:2841852/1‑71 (MQ=255)                                             cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt                       >  1:976624/1‑71 (MQ=255)                                               cccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg                      <  1:1550310/69‑1 (MQ=255)                                                ccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg                      <  1:2304198/69‑1 (MQ=255)                                                ccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg                      <  1:3962262/69‑1 (MQ=255)                                                  ccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAgg                  <  1:3118529/71‑1 (MQ=255)                                                   cTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCa                                       <  1:1583241/49‑1 (MQ=255)                                                     gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt               >  1:1276666/1‑71 (MQ=255)                                                     gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt               >  1:3630264/1‑71 (MQ=255)                                                     gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt               >  1:4404614/1‑71 (MQ=255)                                                     gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt               >  1:3011122/1‑71 (MQ=255)                                                     gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt               >  1:1768010/1‑71 (MQ=255)                                                      ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAc                                         >  1:1358875/1‑44 (MQ=255)                                                      ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAc                                         >  1:3517534/1‑44 (MQ=255)                                                      ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGc                <  1:2111479/69‑1 (MQ=255)                                                          acacCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTAc           >  1:2973912/1‑70 (MQ=255)                                                            acCATCACATATCGCCGGAACACCGGCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa        >  1:2260969/1‑71 (MQ=255)                                                            acCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa        >  1:3575943/1‑71 (MQ=255)                                                            acCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa        >  1:2150150/1‑71 (MQ=255)                                                             ccATCACATATCGCCGGAACACCGCCCGCAACCTGa                                          >  1:1629359/1‑36 (MQ=255)                                                             ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt       <  1:95693/71‑1 (MQ=255)                                                             ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt       <  1:4133072/71‑1 (MQ=255)                                                             ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt       <  1:206330/71‑1 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAtt                               >  1:2855842/1‑45 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATg      >  1:4480394/1‑70 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa     >  1:2583946/1‑71 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa     >  1:2956854/1‑71 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa     >  1:3685181/1‑71 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa     >  1:2131016/1‑71 (MQ=255)                                                               aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGAATTCGCTTCATGCAGGGCTTTACGAATGa     >  1:2154434/1‑71 (MQ=255)                                                                tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt       <  1:2387398/68‑1 (MQ=255)                                                                tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAt    <  1:2247492/71‑1 (MQ=255)                                                                 cacataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCa                        >  1:70587/1‑50 (MQ=255)                                                                 cacataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt   >  1:3471871/1‑71 (MQ=255)                                                                  acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCa                                 >  1:2329057/1‑40 (MQ=255)                                                                  acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAttt  >  1:1627427/1‑71 (MQ=255)                                                                  acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt   >  1:4385612/1‑70 (MQ=255)                                                                  acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt   >  1:3980981/1‑70 (MQ=255)                                                                     |                                                                    ATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT  >  W3110S.gb/1935918‑1936053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑