Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,962,205	C→T	R144C (CGT→TGT)	argS →	arginyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,962,205	0	C	T	100.0%	99.6 / NA	34	R144C (CGT→TGT)	argS	arginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/19);  total (15/19)

GCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTT  >  W3110S.gb/1962138‑1962272                                                                    |                                                                    gcCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTa                                                                  <  1:4535909/71‑1 (MQ=255) gcCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTa                                                                  <  1:3952537/71‑1 (MQ=255)     aaCGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTActct                                                              >  1:3009733/1‑71 (MQ=255)      aCGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTg                                                             <  1:910676/71‑1 (MQ=255)                agagATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCg                                                   >  1:2505114/1‑71 (MQ=255)                      gCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGTAGTTCCTCGGTcac                                              >  1:1141555/1‑70 (MQ=255)                       cATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa                                            <  1:3660115/71‑1 (MQ=255)                           tCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGt                                         >  1:1761690/1‑70 (MQ=255)                           tCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGt                                         <  1:2286602/70‑1 (MQ=255)                             ggTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGAt                                      >  1:1827276/1‑71 (MQ=255)                                cACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTcg                                   >  1:2416020/1‑71 (MQ=255)                                    tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTgg                                                            <  1:1254716/42‑1 (MQ=255)                                    tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAg                                          >  1:1733002/1‑60 (MQ=255)                                    tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCgcgc                                >  1:4030588/1‑70 (MQ=255)                                     gcgcTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCa                               >  1:832638/1‑70 (MQ=255)                                       gcTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAAc                            <  1:2831574/71‑1 (MQ=255)                                           tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa                                            <  1:3614095/51‑1 (MQ=255)                                           tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa                                            <  1:934925/51‑1 (MQ=255)                                           tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCa                          <  1:1661181/69‑1 (MQ=255)                                             ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc                      >  1:326476/1‑71 (MQ=255)                                             ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc                      >  1:2334135/1‑71 (MQ=255)                                             ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc                      >  1:222833/1‑71 (MQ=255)                                             ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc                      >  1:3983333/1‑71 (MQ=255)                                                 tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc                   <  1:4002146/70‑1 (MQ=255)                                                 tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc                   <  1:4447954/70‑1 (MQ=255)                                                 tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc                   <  1:418571/70‑1 (MQ=255)                                                  attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg                  <  1:2122646/70‑1 (MQ=255)                                                  attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg                  <  1:463659/70‑1 (MQ=255)                                                  attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTAGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGa                 >  1:851414/1‑71 (MQ=255)                                                   ttGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg                  <  1:1513424/69‑1 (MQ=255)                                                   ttGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACAACGTCGGCg                  <  1:2563496/69‑1 (MQ=255)                                                     ggTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTg              >  1:1763714/1‑71 (MQ=255)                                                         aCGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTTATTCGCGCAAACCACGTCGGCGACTGGGGc          <  1:3493644/71‑1 (MQ=255)                                                              gcagTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCa     <  1:2317695/71‑1 (MQ=255)                                                                 gtgtgtACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGtt  >  1:1144268/1‑71 (MQ=255)                                                                    |                                                                    GCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTT  >  W3110S.gb/1962138‑1962272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑