Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,000,742	C→T	M292I (ATG→ATA)	yedO ←	D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,000,742	0	C	T	100.0%	140.2 / NA	47	M292I (ATG→ATA)	yedO	D‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/20);  total (27/20)

ATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC  >  W3110S.gb/2000676‑2000804                                                                   |                                                               aTGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg                                                            >  1:4397652/1‑71 (MQ=255)  tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg                                                            >  1:1661508/1‑70 (MQ=255)  tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg                                                            <  1:3095620/70‑1 (MQ=255)    aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt                                                         >  1:3582472/1‑71 (MQ=255)    aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt                                                         >  1:3089062/1‑71 (MQ=255)    aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt                                                         >  1:2808803/1‑71 (MQ=255)     aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCttt                                                        >  1:2794986/1‑71 (MQ=255)        aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTcc                                                     >  1:4624359/1‑71 (MQ=255)                 ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATacac                                            <  1:3944383/71‑1 (MQ=255)                 ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATaca                                             >  1:4462250/1‑70 (MQ=255)                       tCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGtata                                               >  1:1455673/1‑62 (MQ=255)                       tCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGAt                                       >  1:3744620/1‑70 (MQ=255)                        cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATc                                      >  1:74000/1‑70 (MQ=255)                        cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATc                                      >  1:2399458/1‑70 (MQ=255)                            tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGtata                                               >  1:1084957/1‑57 (MQ=255)                            tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg                                   >  1:2836950/1‑69 (MQ=255)                            tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg                                   >  1:21321/1‑69 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGc                                  <  1:1836365/68‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCa                                 <  1:1698671/69‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:688326/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:3183691/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:3269558/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:3309837/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:3643009/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:3809496/70‑1 (MQ=255)                              tGAAGCGTTTCTGACTGATACCGTCAATCAG‑‑CCGCTATCGCTTTTCCGGTATACACAGGATCAAGCa                                 <  1:717044/67‑1 (MQ=255)                                     tttCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGaa                              >  1:3420277/1‑65 (MQ=255)                                       tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                       >  1:3004365/1‑70 (MQ=255)                                       tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                       >  1:3129558/1‑70 (MQ=255)                                       tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc                       >  1:4060271/1‑70 (MQ=255)                                         tgactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                                                   <  1:2947228/40‑1 (MQ=255)                                          gactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAgg                                         <  1:3828615/49‑1 (MQ=255)                                          gactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAgg                                         <  1:4135831/49‑1 (MQ=255)                                           actgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:1711233/57‑1 (MQ=255)                                                tACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg                                <  1:341443/52‑1 (MQ=255)                                                  ccGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg                            <  1:3748257/54‑1 (MQ=255)                                                  ccGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg                            <  1:1738901/54‑1 (MQ=255)                                                     tCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGcc                          >  1:1311075/1‑53 (MQ=255)                                                       aaTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt      >  1:862417/1‑71 (MQ=255)                                                       aaTCAGCCCCGCTATCGCTTTTCCGGTATACAAAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt      >  1:3469743/1‑71 (MQ=255)                                                        aTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt     <  1:3507684/71‑1 (MQ=255)                                                         tCACCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTc    <  1:2487573/71‑1 (MQ=255)                                                          cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa   >  1:4095764/1‑71 (MQ=255)                                                          cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa   >  1:3845030/1‑71 (MQ=255)                                                          cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa   >  1:4647078/1‑71 (MQ=255)                                                          cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa   >  1:2101647/1‑71 (MQ=255)                                                           aGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAc  >  1:2050974/1‑71 (MQ=255)                                                                   |                                                               ATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC  >  W3110S.gb/2000676‑2000804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑