Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,020,305	A→G	T75A (ACG→GCG)	fliJ →	flagellar protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,020,305	0	A	G	100.0%	139.8 / NA	41	T75A (ACG→GCG)	fliJ	flagellar protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)

ACCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACA  >  W3110S.gb/2020237‑2020368                                                                     |                                                                aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCGGCAGTTTATCCAGGCg                                                               >  1:2049228/1‑71 (MQ=255) aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg                                                               >  1:950825/1‑71 (MQ=255) aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg                                                               >  1:1362734/1‑71 (MQ=255) aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg                                                               >  1:3687480/1‑71 (MQ=255)         aGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGaaaa                                                       <  1:81931/71‑1 (MQ=255)            gATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGc                                                    <  1:3571800/71‑1 (MQ=255)                tGAGTGCCGTGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt                                                 <  1:3600472/70‑1 (MQ=255)                tGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt                                                 <  1:3539901/70‑1 (MQ=255)                tGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt                                                 <  1:4481955/70‑1 (MQ=255)                  aGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCAAGGCGCTGGAAAAAGCCATTAc                                              >  1:501314/1‑71 (MQ=255)                           aTAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCg                                     >  1:3518535/1‑71 (MQ=255)                            tAACCAGCAACCGCTGGATCAACTATCAGCAGTTTTTCCAGGCGCTGGAGAAAGCCATTACTCAGCATCGc                                    <  1:3838614/71‑1 (MQ=255)                              aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc                                    >  1:425391/1‑69 (MQ=255)                              aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc                                    >  1:1633899/1‑69 (MQ=255)                              aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc                                    >  1:1423032/1‑69 (MQ=255)                                 aGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa                               <  1:3999292/71‑1 (MQ=255)                                 aGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa                               <  1:1571137/71‑1 (MQ=255)                                        gCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAAt                        <  1:2193497/71‑1 (MQ=255)                                         cTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc                       <  1:1555884/71‑1 (MQ=255)                                         cTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc                       <  1:3167968/71‑1 (MQ=255)                                            gATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAg                                          <  1:3910383/49‑1 (MQ=255)                                            gATCAACTATCAGAAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAg                     <  1:3628409/70‑1 (MQ=255)                                            gATCAACCATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCa                      <  1:287182/69‑1 (MQ=255)                                                   tATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa                               <  1:3370237/53‑1 (MQ=255)                                                   tATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACg               >  1:1412777/1‑69 (MQ=255)                                                    aTCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCa                                        >  1:1820272/1‑43 (MQ=255)                                                       agcagTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGaa          <  1:3812831/70‑1 (MQ=255)                                                           gTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGtt      >  1:2649869/1‑70 (MQ=255)                                                            tttATCCAGGCGCTGGAAAAAGCCATTACTGAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa    <  1:1756976/71‑1 (MQ=255)                                                            tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc                       <  1:2570673/52‑1 (MQ=255)                                                            tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa    <  1:4095117/71‑1 (MQ=255)                                                            tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa    <  1:1648424/71‑1 (MQ=255)                                                             ttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa    >  1:2566092/1‑70 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTaa                         >  1:3596533/1‑48 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTaa                         >  1:3232322/1‑48 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGAc   <  1:3932769/70‑1 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa  >  1:2856976/1‑71 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa  >  1:2879679/1‑71 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa  >  1:4534380/1‑71 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa  >  1:890633/1‑71 (MQ=255)                                                              tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa  >  1:3291792/1‑71 (MQ=255)                                                                     |                                                                ACCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACA  >  W3110S.gb/2020237‑2020368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑