Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,069,625	C→T	intergenic (‑167/‑1147)	insH ← / → yoeA	IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,069,625	0	C	T	100.0%	94.6 / NA	32	intergenic (‑167/‑1147)	insH/yoeA	IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

TTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGG  >  W3110S.gb/2069561‑2069688                                                                 |                                                                tttATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCt                                                            <  1:3887499/70‑1 (MQ=255)   tattaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGa                                                          <  1:3913291/70‑1 (MQ=255)     ttaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGAcat                                                       <  1:4228775/71‑1 (MQ=255)     ttaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGAcat                                                       <  1:2299493/71‑1 (MQ=255)         cgcCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACatcatc                                                   <  1:1089419/71‑1 (MQ=255)                    gTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATaaa                                          >  1:953761/1‑69 (MQ=255)                     tttAACTGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGa                                                          <  1:4684227/52‑1 (MQ=255)                       tAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAgg                                      <  1:695216/70‑1 (MQ=255)                         aCCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGtt                                    <  1:4566565/70‑1 (MQ=255)                           cGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTAt                                 <  1:3422468/71‑1 (MQ=255)                             ggTAACCACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGttt                                   <  1:1510150/67‑1 (MQ=255)                                  acACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGa                                                          >  1:2691356/2‑39 (MQ=255)                                   cACGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACt                           >  1:2730215/1‑69 (MQ=255)                                    aCGACCCAGTCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACGCTCag                        <  1:4335533/71‑1 (MQ=255)                                             tCGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATca                               <  1:4159483/55‑1 (MQ=255)                                              cGGGGATATATTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGttc              >  1:4376292/1‑71 (MQ=255)                                                   atatatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag         <  1:4379099/71‑1 (MQ=255)                                                     atatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcag         >  1:2521018/1‑69 (MQ=255)                                                      tatTCCATCTTTTCGCTGACATCATCAAGTATAAAAAGGTTTAt                                 >  1:3647109/1‑44 (MQ=38)                                                      tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:3410712/1‑71 (MQ=255)                                                      tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:1834269/1‑71 (MQ=255)                                                      tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:448961/1‑71 (MQ=255)                                                      tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:317042/1‑71 (MQ=255)                                                      tatTCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:2488982/1‑71 (MQ=255)                                                      tatTCAATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagcag      >  1:1602043/1‑71 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCaa                   >  1:224071/1‑56 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCaa                   >  1:1040497/1‑56 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTcagca       >  1:2866631/1‑68 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGc    >  1:3940917/1‑71 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGc    >  1:3751542/1‑71 (MQ=255)                                                        ttCCATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGc    >  1:3336491/1‑71 (MQ=255)                                                          ccATCTTTTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCgg  >  1:944108/1‑71 (MQ=255)                                                                 |                                                                TTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGG  >  W3110S.gb/2069561‑2069688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑