BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,074,141	C→T	Q156* (CAG→TAG)	flu →	antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,074,141	0	C	T	100.0%	87.9 / NA	30	Q156* (CAG→TAG)	flu	antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/13); total (17/13)

TTATCAGTGCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCA  >  W3110S.gb/2074073‑2074207

ttATCAGTGCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAg                                                                  >  1:3533079/1‑71 (MQ=255)

 tATCAGTGCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAg                                                                  <  1:1991576/70‑1 (MQ=255)

      gTGCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATg                                                            <  1:420706/71‑1 (MQ=255)

        gCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCa                                                          >  1:1369295/1‑71 (MQ=255)

        gCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCa                                                          >  1:1544303/1‑71 (MQ=255)

        gCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCa                                                          >  1:2383173/1‑71 (MQ=255)

         ccGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCAt                                                         >  1:3752333/1‑71 (MQ=255)

         ccGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCAt                                                         >  1:3108592/1‑71 (MQ=255)

         ccGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCAt                                                         >  1:3012752/1‑71 (MQ=255)

                        gCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTgg                                                               >  1:3723825/1‑50 (MQ=255)

                             cAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAg                                      >  1:3046698/1‑70 (MQ=255)

                              aGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGa                                    <  1:2828939/71‑1 (MQ=255)

                                ggACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATg                                                            <  1:2213338/45‑1 (MQ=255)

                                ggACGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATg                                                            <  1:3016800/45‑1 (MQ=255)

                                  aCGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAcca                                >  1:1326023/1‑70 (MQ=255)

                                  aCGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAcca                                >  1:141404/1‑70 (MQ=255)

                                   cGGGCTGTGAACACCACGCTGAATGTTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAccat                               <  1:1161563/71‑3 (MQ=255)

                                   cGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAccat                               <  1:1747532/71‑3 (MQ=255)

                                   cGGGCTGTGAACACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAccat                               <  1:4020616/71‑3 (MQ=255)

                                            aaCACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGa                      <  1:2607573/71‑1 (MQ=255)

                                            aaCACCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGa                      <  1:3880816/71‑1 (MQ=255)

                                             acacCACGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCAtt                           <  1:722630/65‑1 (MQ=255)

                                                  acGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAggg                >  1:3353554/1‑71 (MQ=255)

                                                  acGCTGAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAggg                >  1:4365567/1‑71 (MQ=255)

                                                       gAATGGTGGCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAAccat                               <  1:780643/51‑3 (MQ=255)

                                                            gtggCGAATAGTGTATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGgtc      >  1:1464507/1‑71 (MQ=255)

                                                            gtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGgtc      >  1:2754740/1‑71 (MQ=255)

                                                            gtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGgtc      >  1:238156/1‑71 (MQ=255)

                                                            gtggCGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGgtc      >  1:941190/1‑71 (MQ=255)

                                                                cGAATAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCATCATTAATGATAAGGGCTGGCAGGTCGTCa  <  1:2512666/71‑1 (MQ=255)

TTATCAGTGCCGGAGGCGGACAGAGCCTTCAGGGACGGGCTGTGAACACCACGCTGAATGGTGGCGAACAGTGGATGCATGAGGGGGCGATAGCCACAGGAACCGTCATTAATGATAAGGGCTGGCAGGTCGTCA  >  W3110S.gb/2074073‑2074207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`