Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	313,430	G→A	intergenic (‑1429/‑151)	ykgM ← / → eaeH	predicted ribosomal protein/attaching and effacing protein, pathogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	313,430	0	G	A	100.0%	38.9 / NA	15	intergenic (‑1429/‑151)	ykgM/eaeH	predicted ribosomal protein/attaching and effacing protein, pathogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

CATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAT  >  W3110S.gb/313374‑313493                                                         |                                                                cATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGAATATATTa                                                         <  1:2724629/65‑1 (MQ=255)   taaataaaAATATAATACCGCGCCCAAACACCTGATGAGTGGCTATAGGCACTGAATATATTAgg                                                       >  1:2333896/1‑65 (MQ=255)     aataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGt                                                >  1:591906/1‑70 (MQ=255)       taaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCa                                             <  1:2406256/71‑1 (MQ=255)              ataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCt                                       >  1:3966890/1‑70 (MQ=255)                       cgcCCAACCACCTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAgg                             <  1:2974154/71‑1 (MQ=255)                         cccAACCACCTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGt                            >  1:1083393/1‑70 (MQ=255)                                accTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCtt                    <  1:3634208/71‑1 (MQ=255)                                  cTGATGAGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCtttt                  >  1:2944621/1‑71 (MQ=255)                                   tgatgaGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAgg                             <  1:1441343/59‑1 (MQ=255)                                    gatgaGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCt                >  1:1050439/1‑71 (MQ=255)                                       gaGTGGCTATAGGCACTGAATATATTAGGTGGCGGTGAACTTTCTTACATAAAGGTATTTCCTTTTCTGc              >  1:4084805/1‑70 (MQ=255)                                                  ggCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAt  >  1:1028963/1‑71 (MQ=255)                                                  ggCACTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAt  >  1:3893794/1‑71 (MQ=255)                                                      cTGAATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTAtt                         <  1:534263/44‑1 (MQ=255)                                                         |                                                                CATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAT  >  W3110S.gb/313374‑313493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑