BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,096,084	G→A	E160E (GAG→GAA)	hisB →	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,096,084	0	G	A	100.0%	387.1 / NA	128	E160E (GAG→GAA)	hisB	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (69/59); total (69/59)

Only 100 of 129 total aligned reads displayed.

GGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC  >  W3110S.gb/2096018‑2096151

ggAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGaacaac                                                                 >  1:1537470/1‑71 (MQ=255)

   aaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCa                                                              >  1:1281386/1‑71 (MQ=255)

   aaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCa                                                              >  1:4628656/1‑71 (MQ=255)

        tGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGa                                                         <  1:1349819/71‑1 (MQ=255)

          ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACg                                                       >  1:3935466/1‑71 (MQ=255)

           gCATTACTGGTTTACGCTACGCCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:563190/1‑71 (MQ=255)

           gCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:1155148/1‑71 (MQ=255)

           gCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:4538004/1‑71 (MQ=255)

              ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAg                                                          <  1:248924/64‑1 (MQ=255)

              ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                                                   <  1:4018981/71‑1 (MQ=255)

                aatGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2220584/3‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACaa                                                                  >  1:2361036/1‑54 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAg                                                          >  1:3306786/1‑62 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGa                                                    >  1:1109946/1‑68 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGa                                                    >  1:3428747/1‑68 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4572759/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4567373/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4391049/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:241880/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:790971/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2454718/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4152401/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3080509/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2734403/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2823573/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2889402/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1821513/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1220774/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1233807/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1083799/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3817616/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1427499/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:678984/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3499483/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3481853/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1583136/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1590012/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:588463/1‑71 (MQ=255)

                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3400629/1‑71 (MQ=255)

                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                <  1:545225/71‑1 (MQ=255)

                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                >  1:3203632/1‑71 (MQ=255)

                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                <  1:3561114/71‑1 (MQ=255)

                    gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTAc                                             <  1:2500236/71‑1 (MQ=255)

                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          >  1:193526/1‑71 (MQ=255)

                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          >  1:1905523/1‑71 (MQ=255)

                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACACACCAGACGTGACCGTTACGCt                                          >  1:109534/1‑71 (MQ=255)

                        aCGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          <  1:3911605/70‑1 (MQ=255)

                        aCGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          <  1:1309032/70‑1 (MQ=255)

                         cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa                                        <  1:3504639/71‑1 (MQ=255)

                         cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa                                        <  1:3740566/71‑1 (MQ=255)

                           cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      <  1:167220/71‑1 (MQ=255)

                            tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:1518713/1‑70 (MQ=255)

                             aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:2874017/1‑69 (MQ=255)

                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:2714383/69‑1 (MQ=255)

                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:3265511/69‑1 (MQ=255)

                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4571096/69‑1 (MQ=255)

                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4631851/69‑1 (MQ=255)

                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:1716877/69‑1 (MQ=255)

                                   gcgAAACCCTGAACTTGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:3853340/71‑1 (MQ=255)

                                   gcgAAACCCTGAACTGGCCATTGATTGGCGAACAACTCTCCAGACGTGACCGTTACGCTCACGTTGTGCGt                              <  1:2559111/71‑1 (MQ=255)

                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:1580315/71‑1 (MQ=255)

                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:1171589/71‑1 (MQ=255)

                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:715144/71‑1 (MQ=255)

                                    cgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                             <  1:1397169/71‑1 (MQ=255)

                                     gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                            <  1:1861660/71‑1 (MQ=255)

                                     gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                             <  1:2981747/70‑1 (MQ=255)

                                              aagtGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                     <  1:3855859/66‑1 (MQ=255)

                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                     <  1:382270/69‑1 (MQ=255)

                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:1700790/71‑1 (MQ=255)

                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:662835/71‑1 (MQ=255)

                                                  ggCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg               >  1:1073105/1‑71 (MQ=255)

                                                   gCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg               >  1:4639623/1‑70 (MQ=255)

                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:1049480/71‑1 (MQ=255)

                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:2040209/71‑1 (MQ=255)

                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:1021349/71‑1 (MQ=255)

                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:364688/71‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:660148/43‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4063174/43‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:412654/43‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTTCGTAATACCAAAGAGACGCAg            <  1:1730508/68‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:148847/61‑1 (MQ=255)

                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg            <  1:2230312/68‑1 (MQ=255)

                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAg                                   >  1:513599/1‑44 (MQ=255)

                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:2455078/1‑53 (MQ=255)

                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt         >  1:3717087/1‑70 (MQ=255)

                                                          aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:3673375/1‑40 (MQ=255)

                                                          aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       >  1:657697/1‑71 (MQ=255)

                                                          aTTGGCGAACAACTCACAAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       >  1:2333351/1‑71 (MQ=255)

                                                           ttGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                            <  1:438522/49‑1 (MQ=255)

                                                           ttGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       <  1:433754/70‑1 (MQ=255)

                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:3385689/45‑1 (MQ=255)

                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:2079906/1‑49 (MQ=255)

                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:4080427/1‑49 (MQ=255)

                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1917572/70‑1 (MQ=255)

                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1912150/70‑1 (MQ=255)

                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1808104/70‑1 (MQ=255)

                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3322708/70‑1 (MQ=255)

                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3595260/70‑1 (MQ=255)

                                                               cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCg                               <  1:2429652/42‑1 (MQ=255)

                                                               cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3505984/69‑1 (MQ=255)

GGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC  >  W3110S.gb/2096018‑2096151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`