Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,172,235 C→T pseudogene (42/447 nt)
pseudogene (1636/2041 nt)
gatR ←
gatR ←
DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,172,2350CT100.0% 268.0 / NA 88pseudogene (42/447 nt)
pseudogene (1636/2041 nt)
gatR
gatR
DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/40);  total (48/40)

CAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAA  >  W3110S.gb/2172167‑2172302
                                                                    |                                                                    
caGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGt                                                                    >  1:333479/1‑71 (MQ=255)
  gacgacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGa                                                                  >  1:817493/1‑71 (MQ=255)
     gacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGatat                                                              <  1:1022906/71‑1 (MQ=255)
        cAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATAtttt                                                           >  1:3689711/1‑71 (MQ=255)
            gTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGc                                                       >  1:2889317/1‑71 (MQ=255)
             tAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCg                                                      >  1:1123992/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:500210/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:615874/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:2968408/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:2546451/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:682558/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    <  1:2388212/71‑1 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:3570523/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:4091765/1‑71 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    <  1:1558942/71‑1 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    <  1:1555322/71‑1 (MQ=255)
               aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    >  1:1089139/1‑71 (MQ=255)
                tGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGa                                                                  <  1:436672/57‑1 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACtgtg                                                                   >  1:3526544/1‑54 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGa                                                                  >  1:900592/1‑55 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCa                                                  >  1:11093/1‑70 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:416900/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:1065189/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:870074/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:4244467/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:2806393/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:1836410/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:3429432/1‑71 (MQ=255)
                  tCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAt                                                 >  1:816441/1‑71 (MQ=255)
                  tCTTAATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCa                                                  >  1:2878275/1‑70 (MQ=255)
                    tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATAtttt                                                           >  1:3542631/1‑59 (MQ=255)
                    tttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAtt                                                >  1:1992863/1‑70 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCTGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAtt                                                <  1:561502/68‑1 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCAtt                                                <  1:489607/68‑1 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              <  1:3242311/70‑1 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              <  1:3221875/70‑1 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAAt                                             >  1:1630142/1‑71 (MQ=255)
                      tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAAt                                             >  1:870116/1‑71 (MQ=255)
                       aTTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTTGGCGGGCATTaa                                              <  1:1398064/70‑1 (MQ=255)
                       aTTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAAt                                             <  1:1109839/70‑1 (MQ=255)
                        ttttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTCGCGGTCATTAATcc                                           <  1:793475/71‑1 (MQ=255)
                                aaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGa                                   >  1:4204638/1‑71 (MQ=255)
                                aaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGa                                   >  1:1152771/1‑71 (MQ=255)
                                aaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGa                                   >  1:1854090/1‑71 (MQ=255)
                                 aGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTTGGCGGTCATTAATCCTTCGGCGa                                   >  1:1022978/1‑71 (MQ=255)
                                  gggCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGt                                                    <  1:557096/52‑1 (MQ=255)
                                   ggCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt                                  >  1:4658396/1‑69 (MQ=255)
                                   ggCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt                                  >  1:328478/1‑69 (MQ=255)
                                   ggCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGAt                                  >  1:1144236/1‑69 (MQ=255)
                                   ggCAAACGCTGCCGGGAGACTGTTGCTAATCACTGTGatat                                                              >  1:539028/1‑41 (MQ=255)
                                    gCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              >  1:1696465/1‑56 (MQ=255)
                                    gCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               <  1:3562393/71‑1 (MQ=255)
                                    gCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               >  1:1419451/1‑71 (MQ=255)
                                    gCAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               <  1:2023625/71‑1 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCa                                                  >  1:75536/1‑51 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               >  1:145795/1‑70 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc                              <  1:2170225/71‑1 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc                              <  1:3437904/71‑1 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc                              <  1:1592283/71‑1 (MQ=255)
                                     cAAACGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGCCGATAAGc                              <  1:3619500/71‑1 (MQ=255)
                                       aaCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt                            <  1:270386/71‑1 (MQ=255)
                                       aaCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt                            <  1:4398607/71‑1 (MQ=255)
                                       aaCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt                            <  1:4051309/71‑1 (MQ=255)
                                       aaCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt                            <  1:2434564/71‑1 (MQ=255)
                                       aaCGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCAt                            <  1:279515/71‑1 (MQ=255)
                                         cGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              <  1:1642275/51‑1 (MQ=255)
                                         cGCTGCCGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              <  1:606322/51‑1 (MQ=255)
                                              ccGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCg                       <  1:1990465/69‑1 (MQ=255)
                                              ccGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCg                       <  1:105869/69‑1 (MQ=255)
                                              ccGGGAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCg                       <  1:530484/69‑1 (MQ=255)
                                                 ggAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               <  1:1227727/58‑1 (MQ=255)
                                                 ggAGACTGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAg                               <  1:4500325/58‑1 (MQ=255)
                                                       tGTTGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCa                             >  1:992952/1‑54 (MQ=255)
                                                        gTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              >  1:2510445/1‑36 (MQ=255)
                                                        gTTGGTAATCACTGTGATATTTTTGGCGGTCATTaa                                              >  1:2005323/1‑36 (MQ=255)
                                                        gTTGGTAATCACTGTGATATTTTTGGGGTCATTaa                                              >  1:3951227/1‑35 (MQ=25)
                                                         ttGGTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCt             <  1:1551510/68‑1 (MQ=255)
                                                           ggTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTc          >  1:3673889/1‑69 (MQ=255)
                                                            gTAATCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCgag       <  1:539566/71‑1 (MQ=255)
                                                              aaTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTc                 >  1:1068364/1‑59 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGaa     >  1:2396948/1‑70 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt    <  1:3845675/71‑1 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt    <  1:587985/71‑1 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt    <  1:1383021/71‑1 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt    <  1:1322007/71‑1 (MQ=255)
                                                               aTCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAAt    <  1:1212213/71‑1 (MQ=255)
                                                                tCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc                              <  1:320674/44‑1 (MQ=255)
                                                                tCACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGc                              <  1:2229200/44‑1 (MQ=255)
                                                                 cACTGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATaa  >  1:4216455/1‑71 (MQ=255)
                                                                    |                                                                    
CAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAA  >  W3110S.gb/2172167‑2172302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: