Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,204,561	G→A	D317N (GAT→AAT)	yehI →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,204,561	0	G	A	100.0%	140.2 / NA	48	D317N (GAT→AAT)	yehI	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

TGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCA  >  W3110S.gb/2204498‑2204616                                                                |                                                        tGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATaaaaa                                                  >  1:1191230/1‑71 (MQ=255)     gCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaacaaa                                              <  1:695309/71‑1 (MQ=255)     gCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaacaaa                                              <  1:4566983/71‑1 (MQ=255)        gTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaaca                                                <  1:4364862/66‑1 (MQ=255)        gTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaaca                                                <  1:270930/66‑1 (MQ=255)         tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCa                                          <  1:4135132/71‑1 (MQ=255)          ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg                                         >  1:3675089/1‑71 (MQ=255)          ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg                                         >  1:4598939/1‑71 (MQ=255)          ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg                                         >  1:1926963/1‑71 (MQ=255)          ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg                                         >  1:537486/1‑71 (MQ=255)           tgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGc                                        >  1:1090551/1‑71 (MQ=255)                gTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGc                                    <  1:2310311/70‑1 (MQ=255)                     aaTTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg                              >  1:491420/1‑71 (MQ=255)                      aTTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtgt                             <  1:2750424/71‑1 (MQ=255)                      aTTAACCATCCGCAAGTCATCCGAACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtgt                             <  1:4260772/71‑1 (MQ=255)                       ttAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg                              >  1:3031209/1‑69 (MQ=255)                       ttAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg                              >  1:3075084/1‑69 (MQ=255)                           ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                        <  1:3643191/71‑1 (MQ=255)                           ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                        <  1:1705271/71‑1 (MQ=255)                           ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                        <  1:131203/71‑1 (MQ=255)                            cATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                        <  1:2491286/70‑1 (MQ=255)                               ccGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                    >  1:1211650/1‑71 (MQ=255)                                cGCAAGTCATCCGCACTTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   >  1:3856161/1‑71 (MQ=255)                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   >  1:3497923/1‑71 (MQ=255)                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   <  1:4072608/71‑1 (MQ=255)                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   >  1:2809145/1‑71 (MQ=255)                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   <  1:2485467/71‑1 (MQ=255)                                cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                   >  1:503103/1‑71 (MQ=255)                                  cAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg                              <  1:4687295/58‑1 (MQ=255)                                      tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaa             <  1:3067467/71‑1 (MQ=255)                                      tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaa             <  1:455195/71‑1 (MQ=255)                                      tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACACAGCCTGCAACGTGTAGCTAAATACCATaaa             <  1:1479773/71‑1 (MQ=255)                                        aTCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaa            <  1:2481964/70‑1 (MQ=255)                                         tCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCAt                <  1:2048958/65‑1 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAg                           >  1:881879/1‑52 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                    >  1:3452613/1‑59 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa              >  1:3038261/1‑65 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt        >  1:2774476/1‑71 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt        >  1:1458973/1‑71 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt        >  1:1123862/1‑71 (MQ=255)                                           cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACt         <  1:2688687/70‑1 (MQ=255)                                            gCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTc       <  1:4149929/71‑1 (MQ=255)                                               ccTTGCTACTGGTGGCTAATAAAAACAAACCCATCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc    <  1:1437889/71‑1 (MQ=255)                                               ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc    <  1:3415391/71‑1 (MQ=255)                                               ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc    <  1:198939/71‑1 (MQ=255)                                               ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc    <  1:1543100/71‑1 (MQ=255)                                                cTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                        <  1:1975717/50‑1 (MQ=255)                                                 ttGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCa  >  1:2232860/1‑71 (MQ=255)                                                                |                                                        TGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCA  >  W3110S.gb/2204498‑2204616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑