Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,215,186	A→G	V115V (GTT→GTC)	yehS ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,215,186	0	A	G	100.0%	162.7 / NA	47	V115V (GTT→GTC)	yehS	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/31);  total (16/31)

GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGC  >  W3110S.gb/2215123‑2215245                                                                |                                                            gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCTTCGCTGTAATTTCCGGCATCGAGACGCgg                                                       <  1:2158736/70‑1 (MQ=255) gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg                                                       <  1:709359/70‑1 (MQ=255) gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg                                                       <  1:339732/70‑1 (MQ=255) gccgcATTCGCGGAAGTTTTTATGATCCGGTGCCCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg                                                       <  1:4247131/70‑1 (MQ=255)     cATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActg                                                  <  1:2246411/71‑1 (MQ=255)       ttCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActgct                                                >  1:3902365/1‑71 (MQ=255)       ttCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActgct                                                >  1:3688787/1‑71 (MQ=255)         cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt                                              >  1:3157117/1‑71 (MQ=255)         cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt                                              >  1:2756701/1‑71 (MQ=255)          gcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGtt                                             <  1:4094185/71‑1 (MQ=255)                 tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg                                      <  1:2439224/71‑1 (MQ=255)                  ttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg                                      >  1:1029429/1‑70 (MQ=255)                    ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGATGCGGAAGTGCTGTTCGGTGAGGa                                    <  1:3171736/70‑1 (MQ=255)                    ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGa                                    <  1:772874/70‑1 (MQ=255)                      aTGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATa                                  <  1:4209337/70‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:3756962/71‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:3670485/71‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:938909/71‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:2984815/71‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:3009955/71‑1 (MQ=255)                          tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg                             <  1:2142744/71‑1 (MQ=255)                                      atcatcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGt                 >  1:4130080/1‑71 (MQ=255)                                        catcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGttt               >  1:945430/1‑71 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCATaa                           >  1:713320/1‑58 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGaa                           >  1:3224148/1‑58 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGaa                           >  1:2253374/1‑58 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt              >  1:2717586/1‑71 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt              >  1:2496609/1‑71 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt              >  1:3899336/1‑71 (MQ=255)                                         atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt              >  1:152700/1‑71 (MQ=255)                                                tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGt                        <  1:1589268/54‑1 (MQ=255)                                                tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa       <  1:2239151/71‑1 (MQ=255)                                                tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa       <  1:1729736/71‑1 (MQ=255)                                                tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa       <  1:758026/71‑1 (MQ=255)                                                tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa       <  1:1092063/71‑1 (MQ=255)                                                     tCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  >  1:3711038/1‑71 (MQ=255)                                                     tCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  >  1:60648/1‑71 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:898011/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:3316651/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:626524/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:4573351/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:4511912/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:2717468/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:1962617/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:1395916/70‑1 (MQ=255)                                                      ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:1038344/70‑1 (MQ=255)                                                      ccGGCAGCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc  <  1:4101728/70‑1 (MQ=255)                                                                |                                                            GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGC  >  W3110S.gb/2215123‑2215245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑