BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,264,229	C→T	L172L (CTG→CTA)	fruA ←	fused fructose‑specific PTS enzyme IIB'BC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,264,229	0	C	T	100.0%	196.2 / NA	66	L172L (CTG→CTA)	fruA	fused fructose‑specific PTS enzyme IIB'BC components
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/38); total (28/38)

CCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAT  >  W3110S.gb/2264162‑2264294

ccTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAg                                                                 <  1:182688/70‑1 (MQ=255)

    cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc                                                              <  1:2315985/69‑1 (MQ=255)

    cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc                                                              <  1:3611109/69‑1 (MQ=255)

    cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc                                                              <  1:879763/69‑1 (MQ=255)

    cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc                                                              <  1:658643/69‑1 (MQ=255)

        gTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc                                                        <  1:3776771/71‑1 (MQ=255)

        gTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc                                                        <  1:4083766/71‑1 (MQ=255)

         ttttCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc                                                        >  1:3827428/1‑70 (MQ=255)

           ttcttcAGCGCCAGACCGGTAGATGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      <  1:1493091/70‑1 (MQ=255)

           ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      >  1:1416016/1‑70 (MQ=255)

           ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      <  1:1494280/70‑1 (MQ=255)

           ctcttcAGCGCCAGACCGTTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      <  1:2535387/69‑1 (MQ=255)

           ctcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      <  1:705636/69‑1 (MQ=255)

           ctcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      <  1:3468555/69‑1 (MQ=255)

            tcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat                                                     <  1:3871544/70‑1 (MQ=255)

            tcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat                                                     <  1:1285638/70‑1 (MQ=255)

                        gACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAg                                                                 <  1:791508/46‑1 (MQ=255)

                          ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc                                                  >  1:4071265/1‑59 (MQ=255)

                          ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt                                      <  1:88084/71‑1 (MQ=255)

                          ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt                                      <  1:3855062/71‑1 (MQ=255)

                                 agGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc                                                        <  1:3544447/46‑1 (MQ=255)

                                 agGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGa                               <  1:371479/71‑1 (MQ=255)

                                  ggTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCAc                                   >  1:1760950/1‑66 (MQ=255)

                                  ggTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCAc                                   >  1:3312405/1‑66 (MQ=255)

                                   gTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      >  1:899104/1‑46 (MQ=255)

                                   gTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATc                             >  1:2413145/1‑71 (MQ=255)

                                    tACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTAGata                                                    <  1:2999242/47‑1 (MQ=255)

                                     aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt                                      >  1:3742523/1‑60 (MQ=255)

                                     aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg                           >  1:4581754/1‑71 (MQ=255)

                                     aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg                           >  1:4587049/1‑71 (MQ=255)

                                     aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg                           >  1:1280626/1‑71 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat                                                     <  1:954435/44‑1 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat                                                     <  1:318943/44‑1 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      >  1:2319518/1‑43 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc                                                  >  1:2814350/1‑47 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc                                                  >  1:1626559/1‑47 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc                          <  1:2834426/71‑1 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc                          >  1:143830/1‑71 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc                          >  1:1570828/1‑71 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc                          >  1:1905570/1‑71 (MQ=255)

                                      cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg                           >  1:1347667/1‑70 (MQ=255)

                                       gATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgct                         <  1:1294390/71‑1 (MQ=255)

                                          aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa                                                      >  1:286020/1‑39 (MQ=255)

                                          aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgctgct                      >  1:4541317/1‑71 (MQ=255)

                                          aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgctgct                      >  1:1670705/1‑71 (MQ=255)

                                           cATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGctgctg                     >  1:385475/1‑71 (MQ=255)

                                            aTCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCTCCAGATCCGCtgctgc                    <  1:101052/71‑1 (MQ=255)

                                            aTCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCtgctgc                    <  1:4611239/71‑1 (MQ=255)

                                             tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc                                                  >  1:2711447/1‑40 (MQ=255)

                                             tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                   <  1:1674317/71‑1 (MQ=255)

                                             tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg                   <  1:2269955/71‑1 (MQ=255)

                                               ggTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc                 >  1:1642635/1‑71 (MQ=255)

                                                 tttACGAGCAAATTTCGCTAGATCCACTTGGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGActt               <  1:626206/71‑1 (MQ=255)

                                                   tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa                                       >  1:2059541/1‑45 (MQ=255)

                                                   tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa                                       >  1:2054234/1‑45 (MQ=255)

                                                   tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa                                       >  1:1323257/1‑45 (MQ=255)

                                                        gCAAATTTCGCTGGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg        <  1:4663681/71‑1 (MQ=255)

                                                        gCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg        <  1:1291803/71‑1 (MQ=255)

                                                         cAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg        <  1:4492952/70‑1 (MQ=255)

                                                           aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt     <  1:2460174/71‑1 (MQ=255)

                                                           aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt     <  1:4490944/71‑1 (MQ=255)

                                                           aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt     <  1:3237751/71‑1 (MQ=255)

                                                            aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg    <  1:689042/71‑1 (MQ=255)

                                                            aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg    <  1:1014266/71‑1 (MQ=255)

                                                            aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg    <  1:2889321/71‑1 (MQ=255)

                                                              ttCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAt  >  1:667138/1‑71 (MQ=255)

CCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAT  >  W3110S.gb/2264162‑2264294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`