Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,285,913	T→C	R21R (CGT→CGC)	rplY →	50S ribosomal subunit protein L25

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,285,913	0	T	C	100.0%	81.1 / NA	26	R21R (CGT→CGC)	rplY	50S ribosomal subunit protein L25
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

GTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGC  >  W3110S.gb/2285853‑2285974                                                             |                                                              gTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAAc                                                      >  1:556107/1‑70 (MQ=255)  tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTaa                                                       >  1:2381852/1‑68 (MQ=255)  tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAAc                                                      >  1:2960155/1‑69 (MQ=255)  tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACa                                                     <  1:3727966/70‑1 (MQ=255)    tACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAg                                                   <  1:1893742/70‑1 (MQ=255)      cTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGtt                                                 <  1:4456418/70‑1 (MQ=255)         tCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTc                                                >  1:649938/1‑68 (MQ=255)         tCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCg                                             >  1:551694/1‑71 (MQ=255)           aaCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCgg                                            >  1:4121241/1‑70 (MQ=255)            aCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCa                                          <  1:2079575/71‑1 (MQ=255)               cAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatc                                       <  1:743812/71‑1 (MQ=255)                aGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatca                                      <  1:2707760/71‑1 (MQ=255)                    gTACGTAAAGAGCAGGGTAAGGTTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTa                                  <  1:997250/71‑1 (MQ=255)                    gTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTa                                  >  1:539427/1‑71 (MQ=255)                     tACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:3754485/71‑1 (MQ=255)                      aCGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACg                                >  1:2203292/1‑71 (MQ=255)                           aaGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCa                                          <  1:2110143/56‑1 (MQ=255)                           aaGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTgg                            >  1:2569274/1‑70 (MQ=255)                                cAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatca                                      <  1:3211188/55‑1 (MQ=255)                                    gTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAgcg                  >  1:1785656/1‑71 (MQ=255)                                       aGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGcgccg               >  1:1058780/1‑71 (MQ=255)                                          gTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:1508047/50‑1 (MQ=255)                                           tGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:3981145/49‑1 (MQ=255)                                                  cgccgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCg     <  1:1001972/70‑1 (MQ=255)                                                   gccgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAg   >  1:3731114/1‑71 (MQ=255)                                                     cgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGc  <  1:219536/70‑1 (MQ=255)                                                             |                                                              GTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGC  >  W3110S.gb/2285853‑2285974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑