BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,295,693	C→T	A18T (GCG→ACG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,295,693	0	C	T	100.0%	295.8 / NA	101	A18T (GCG→ACG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (47/54); total (47/54)

Only 100 of 103 total aligned reads displayed.

CTTCAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTA  >  W3110S.gb/2295627‑2295759

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACATATCGATGGTCGTCAgc                                                                >  1:2855736/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:1066305/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:2874573/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:3966847/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:494801/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:524981/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:3832660/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:568901/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:598899/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:3648880/1‑71 (MQ=255)

cttcAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAgc                                                                >  1:369055/1‑71 (MQ=255)

     gTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGa                                                           <  1:1547864/71‑1 (MQ=255)

     gTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGa                                                           <  1:4488508/71‑1 (MQ=255)

       gAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGc                                                         >  1:625472/1‑71 (MQ=255)

        aGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGc                                                         >  1:906496/1‑70 (MQ=255)

            gACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCTATGGTCGTCAGCGCTGAGCCg                                                       >  1:4353444/1‑68 (MQ=255)

            gACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCg                                                       >  1:219167/1‑68 (MQ=255)

             aCGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACTCATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:1284153/70‑1 (MQ=255)

             aCGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgaga                                                   <  1:3096850/71‑1 (MQ=255)

             aCGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:3453086/70‑1 (MQ=255)

             aCGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:3753094/70‑1 (MQ=255)

             aCGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:2584291/70‑1 (MQ=255)

              cGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  <  1:2021146/71‑1 (MQ=255)

              cGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  <  1:4619757/71‑1 (MQ=255)

               ggAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  >  1:4376376/1‑70 (MQ=255)

               ggAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  >  1:4456596/1‑70 (MQ=255)

                  aCTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatcat                                               >  1:1247945/1‑70 (MQ=255)

                   cTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:3704568/64‑1 (MQ=255)

                   cTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:4356701/64‑1 (MQ=255)

                   cTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatcat                                               >  1:3235081/1‑69 (MQ=255)

                     gttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag                                            >  1:1455963/1‑70 (MQ=255)

                      ttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCGGCGCTGAGCCGGAGATcatcag                                            <  1:2745938/69‑1 (MQ=255)

                      ttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagca                                          <  1:4428926/71‑1 (MQ=255)

                      ttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagc                                           <  1:3731486/70‑1 (MQ=255)

                      ttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag                                            <  1:4409172/69‑1 (MQ=255)

                      ttgttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcag                                            <  1:4608949/69‑1 (MQ=255)

                        gttCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag                                                    <  1:4041156/59‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGGAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:4437033/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  >  1:2613769/1‑58 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  >  1:1034426/1‑58 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:2634501/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:631226/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:620113/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:1659554/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:551969/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:3934881/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:492612/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:346451/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:2100889/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:2244868/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:4608153/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:4471005/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:2478895/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:4384192/70‑1 (MQ=255)

                           ccTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:4455239/70‑1 (MQ=255)

                            cTGTGCTTCATCTTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:752154/70‑1 (MQ=255)

                            cTGTGCTTCATCTTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:3508740/70‑1 (MQ=255)

                            cTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:280151/69‑1 (MQ=255)

                             tgtgCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:3499596/68‑1 (MQ=255)

                               tgCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatca                                                >  1:2985897/1‑56 (MQ=255)

                               tgCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcat                                         >  1:2283358/1‑63 (MQ=255)

                               tgCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGc                                 >  1:3491694/1‑71 (MQ=255)

                               tgCTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGc                                 >  1:2899198/1‑71 (MQ=255)

                                 cTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGcc                                >  1:2448002/1‑70 (MQ=255)

                                 cTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGcc                                >  1:3292375/1‑70 (MQ=255)

                                 cTTCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGAACTGAGCCGGAGATcatcagca                                          >  1:3058603/1‑60 (MQ=255)

                                   tCATC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCaa                             >  1:2100737/1‑71 (MQ=255)

                                      tC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGat                                                  <  1:554385/47‑1 (MQ=255)

                                      tC‑TTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      <  1:2352116/59‑1 (MQ=255)

                                         tttAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagca                                          >  1:2086413/1‑53 (MQ=255)

                                         tttAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         >  1:3747584/1‑70 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatca                                                >  1:617589/1‑45 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatca                                                >  1:4588693/1‑45 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaaa                        <  1:423583/69‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaaa                        <  1:1668415/69‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:198188/68‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:1929390/68‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:629337/68‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:947735/68‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:2887497/68‑1 (MQ=255)

                                           tAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaa                         <  1:4185444/68‑1 (MQ=255)

                                             aaCTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCt                    <  1:4395728/71‑1 (MQ=255)

                                                tGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      >  1:1280042/1‑50 (MQ=255)

                                                tGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagcatcag                                      >  1:3481502/1‑50 (MQ=255)

                                                tGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCAt                 >  1:3747529/1‑71 (MQ=255)

                                                tGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCAt                 >  1:2074717/1‑71 (MQ=255)

                                                 gCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCAtt                >  1:4593373/1‑71 (MQ=255)

                                                   aaCACATCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACCCCCAATAAAAACCTCATtgt              <  1:1201595/71‑1 (MQ=255)

                                                     cacaTCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagc                                           <  1:4199383/40‑1 (MQ=255)

                                                     cacaTCGATGGTCGTCAGCGCTGAGCCGGAGATcatcagc                                           <  1:463103/40‑1 (MQ=255)

                                                      acaTCGATGGTCGTCAGCGCTGAGCCGGAGATCAtcagcatcagca                                    >  1:1573289/1‑46 (MQ=255)

                                                      acaTCGATGGTCGTCAGCGCTGAGCCGGAGATCAtcagcatcagca                                    >  1:1370137/1‑46 (MQ=255)

                                                      acaTCGATGGTCGTCAGCGCTGAGCCGGAGATCAtcagaatcagca                                    >  1:3654179/1‑46 (MQ=255)

                                                       caTCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaaaa                       >  1:1815307/1‑58 (MQ=255)

                                                         tCGATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGcc        <  1:1107892/71‑1 (MQ=255)

                                                           gATGGTCGTCAGCGCTGAGCCGGAGATCAtcaccatcagcag                                   >  1:3648826/1‑41 (MQ=38)

                                                           gATGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATaaaaa                       >  1:2333556/1‑54 (MQ=255)

                                                             tGGTCGTCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGcctcct    <  1:2989839/71‑1 (MQ=255)

                                                               gtcgtcAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTa  <  1:3022485/71‑1 (MQ=255)

                                                               gtcgtcAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTa  <  1:1903732/71‑1 (MQ=255)

CTTCAGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATC‑TTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCATCAGCATCAGCACGCCCAATAAAAACCTCATTGTGCGGCCTCCTTA  >  W3110S.gb/2295627‑2295759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`