Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	408,996	G→A	A83V (GCG→GTG)	rdgC ←	DNA‑binding protein, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	408,996	0	G	A	100.0%	107.2 / NA	36	A83V (GCG→GTG)	rdgC	DNA‑binding protein, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/24);  total (12/24)

AATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCA  >  W3110S.gb/408928‑409061                                                                     |                                                                  aaTCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCAcc                                                                 <  1:3222431/71‑1 (MQ=255)    cTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCAcc                                                                 >  1:135596/1‑68 (MQ=255)       tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt                                                            <  1:3112743/70‑1 (MQ=255)       tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt                                                            <  1:3107413/70‑1 (MQ=255)       tttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt                                                            <  1:3067980/70‑1 (MQ=255)        ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa                                                           <  1:1091455/70‑1 (MQ=255)        ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa                                                           <  1:1210372/70‑1 (MQ=255)        ttCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa                                                           <  1:282686/70‑1 (MQ=255)          cGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt                                                         <  1:943411/70‑1 (MQ=255)                 cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga                                                  <  1:4391305/70‑1 (MQ=255)                 cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga                                                  <  1:2044786/70‑1 (MQ=255)                 cttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGga                                                  <  1:2306210/70‑1 (MQ=255)                         tACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGggag                                          >  1:2987962/1‑70 (MQ=255)                               ccTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGAttttt                                   <  1:20047/71‑1 (MQ=255)                                cTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGttt                                                            <  1:3319135/45‑1 (MQ=255)                                   ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt                                                         <  1:1443109/45‑1 (MQ=255)                                   ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAAt                                                         <  1:1526217/45‑1 (MQ=255)                                   ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATc                                                        <  1:2526994/46‑1 (MQ=255)                                   ttCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATc                                                        <  1:2779431/46‑1 (MQ=255)                                    tCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTttcttc                              >  1:4073900/1‑71 (MQ=255)                                     ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTaa                                                          >  1:2134815/1‑42 (MQ=255)                                     ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTtcttct                             >  1:102235/1‑71 (MQ=255)                                     ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTtcttct                             >  1:1548515/1‑71 (MQ=255)                                              gTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg                    <  1:2417395/71‑1 (MQ=255)                                                ttGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGgaga                                                >  1:3328629/1‑41 (MQ=255)                                                ttGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg                    >  1:915602/1‑69 (MQ=255)                                                 tGGCGATTTTCGCTTCCAGCACCTGTTTATTCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg                    >  1:408101/1‑68 (MQ=255)                                                 tGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcgcg                    >  1:2108089/1‑68 (MQ=255)                                                               tCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc    >  1:1746563/1‑70 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc    <  1:1337746/69‑1 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc    <  1:1239983/69‑1 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc    <  1:2252709/69‑1 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc    <  1:1750618/69‑1 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa  >  1:3141611/1‑71 (MQ=255)                                                                ccAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGAGCAGATAACAATTTGAc    <  1:4515093/69‑1 (MQ=255)                                                                 cAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa  <  1:2324133/70‑1 (MQ=255)                                                                     |                                                                  AATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCA  >  W3110S.gb/408928‑409061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑