Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,307,310	C→T	V24V (GTC→GTT)	eco →	ecotin, a serine protease inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,307,310	0	C	T	100.0%	89.5 / NA	31	V24V (GTC→GTT)	eco	ecotin, a serine protease inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

TCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCA  >  W3110S.gb/2307250‑2307375                                                             |                                                                  tCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTg                                                          >  1:3128110/1‑70 (MQ=255)     ccTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTTGGCGGCAGAAAGCGTTCAGCCACTGGaaaa                                                     <  1:3234867/71‑1 (MQ=255)     ccTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGaaaa                                                     <  1:1128518/71‑1 (MQ=255)     ccTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGaaaa                                                     <  1:3703400/71‑1 (MQ=255)       tGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTTGGCGGCAGAAAGCGTTCAGCCACTGGaaaaa                                                    <  1:2173196/70‑1 (MQ=255)             aTTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCgcgc                                             >  1:1544706/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:966508/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:936987/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:4154360/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:3679012/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:3211437/1‑71 (MQ=255)              ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc                                            >  1:1688679/1‑71 (MQ=255)               tGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCt                                           >  1:760139/1‑71 (MQ=255)                   tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATc                                       >  1:1109050/1‑71 (MQ=255)                          ttCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAg                                 >  1:1436970/1‑70 (MQ=255)                          ttCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGc                                >  1:3907096/1‑71 (MQ=255)                                aCCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATc                                       >  1:2052415/1‑58 (MQ=255)                                 ccACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGaaa                           <  1:228857/69‑1 (MQ=255)                                   aCTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg                       <  1:1031837/71‑1 (MQ=255)                                   aCTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg                       <  1:887172/71‑1 (MQ=255)                                   aCTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg                       <  1:4288846/71‑1 (MQ=255)                                       ccGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGa                     <  1:3810307/69‑1 (MQ=255)                                           cTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGc               <  1:4369280/71‑1 (MQ=255)                                            tGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGc               <  1:1818419/70‑1 (MQ=255)                                                    aGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg                       <  1:2986172/54‑1 (MQ=255)                                                    aGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGa                     <  1:634775/56‑1 (MQ=255)                                                       aaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCca                                     >  1:2425333/1‑37 (MQ=255)                                                       aaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGAtt    >  1:2903166/1‑70 (MQ=255)                                                       aaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGAtt    >  1:240156/1‑70 (MQ=255)                                                        aGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGAtt    >  1:3810741/1‑69 (MQ=255)                                                        aGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCa  <  1:115424/71‑1 (MQ=255)                                                             |                                                                  TCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCA  >  W3110S.gb/2307250‑2307375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑