BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,416,039	G→A	R254C (CGT→TGT)	yfbS ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,416,039	0	G	A	100.0%	198.2 / NA	66	R254C (CGT→TGT)	yfbS	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/35); total (31/35)

AACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAC  >  W3110S.gb/2415974‑2416104

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:4138784/1‑71 (MQ=255)

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:3291773/1‑71 (MQ=255)

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:3413788/1‑71 (MQ=255)

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:4562970/1‑71 (MQ=255)

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:3699931/1‑71 (MQ=255)

aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc                                                              >  1:2122311/1‑71 (MQ=255)

      cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCTATGACGTTAGCGCCATAACACTCACGTAAtt                                                        >  1:1985570/1‑71 (MQ=255)

      cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt                                                        >  1:1476351/1‑71 (MQ=255)

      cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt                                                        >  1:601735/1‑71 (MQ=255)

      cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt                                                        >  1:181121/1‑71 (MQ=255)

      cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt                                                        >  1:1691002/1‑71 (MQ=255)

         gATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTga                                                     <  1:2540016/71‑1 (MQ=255)

           tAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTgag                                                    <  1:1286014/70‑1 (MQ=255)

            aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt                                                       >  1:1271025/1‑66 (MQ=255)

            aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt                                                       >  1:4504179/1‑66 (MQ=255)

            aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt                                                       >  1:3471654/1‑66 (MQ=255)

             aCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGat                                                  <  1:4176172/70‑1 (MQ=255)

              cgcgACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGatca                                                <  1:3105887/71‑1 (MQ=255)

                cgacgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCTCCATAACACTCACGTAATTTGAGatcatc                                              <  1:441381/71‑1 (MQ=255)

                cgacgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGatcatc                                              <  1:4474052/71‑1 (MQ=255)

                  acgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCa                                            <  1:2289601/71‑1 (MQ=255)

                     aaaaCGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGcc                                         <  1:4560930/71‑1 (MQ=255)

                     aaaaCGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGcc                                         <  1:1393670/71‑1 (MQ=255)

                             gccAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCa                                 <  1:65751/71‑1 (MQ=255)

                               cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               <  1:1386532/71‑1 (MQ=255)

                               cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               <  1:3604258/71‑1 (MQ=255)

                               cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               <  1:639554/71‑1 (MQ=255)

                               cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               <  1:1871694/71‑1 (MQ=255)

                                aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               >  1:938742/1‑70 (MQ=255)

                                aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt                               >  1:3673247/1‑70 (MQ=255)

                                aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCACCCAGCCGTTGACCAAt                               >  1:172109/1‑70 (MQ=255)

                                   gTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCAtt                           <  1:1683185/71‑1 (MQ=255)

                                      cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                >  1:4673669/1‑63 (MQ=255)

                                      cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                >  1:858149/1‑63 (MQ=255)

                                      cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGc                        >  1:1171316/1‑71 (MQ=255)

                                        acacCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGa                      <  1:3404643/71‑1 (MQ=255)

                                          acagATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGAt                     >  1:18221/4‑70 (MQ=255)

                                          acCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATc                    >  1:2307719/1‑71 (MQ=255)

                                          acCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATc                    >  1:230622/1‑71 (MQ=255)

                                           ccGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                   >  1:3430456/1‑71 (MQ=255)

                                           ccGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                   >  1:2482209/1‑71 (MQ=255)

                                             gATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg                 >  1:1630447/1‑71 (MQ=255)

                                                 aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:2738950/71‑1 (MQ=255)

                                                 aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:3689952/71‑1 (MQ=255)

                                                 aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:3696393/71‑1 (MQ=255)

                                                 aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:2236187/71‑1 (MQ=255)

                                                  cGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:3248351/70‑1 (MQ=255)

                                                   gTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:2104882/69‑1 (MQ=255)

                                                    ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCa                             <  1:1220300/52‑1 (MQ=255)

                                                    ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCa                             <  1:1959018/52‑1 (MQ=255)

                                                    ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg             <  1:2602981/68‑1 (MQ=255)

                                                    ttAGCGCCATAACACTCACCTAATTTGAGATCATCCAGCCGTTGACCAATCa                             <  1:549864/52‑1 (MQ=255)

                                                      aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg        >  1:1587019/1‑71 (MQ=255)

                                                      aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg        >  1:3526051/1‑71 (MQ=255)

                                                      aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg        >  1:2794009/1‑71 (MQ=255)

                                                      aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg        >  1:2664060/1‑71 (MQ=255)

                                                       gcgcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGc       <  1:4093483/71‑1 (MQ=255)

                                                         gcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGAcc                                  <  1:3708806/42‑1 (MQ=255)

                                                         gcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa     <  1:2555918/71‑1 (MQ=255)

                                                          ccATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGc       <  1:2540930/68‑1 (MQ=255)

                                                          ccATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa     <  1:166500/70‑1 (MQ=255)

                                                            aTAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc  <  1:982353/71‑1 (MQ=255)

                                                            aTAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc  <  1:3999634/71‑1 (MQ=255)

                                                             tAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa     <  1:640192/67‑1 (MQ=255)

                                                             tAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa     <  1:370716/67‑1 (MQ=255)

                                                              aaCACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGaa           >  1:3868210/1‑60 (MQ=255)

AACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAC  >  W3110S.gb/2415974‑2416104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`